Cryoelectron Tomography or Doing Structural Biology In Situ

  • Wolfgang Baumeister
Conference paper
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)


Electron tomography enables the three-dimensional visualization of large and stochastically variable structures such as supramolecular assemblies, organelles or even cells. In conjunction with cryogenic techniques electron tomography avoids the artefacts that are notorious to conventional electron microscopy specimen preparation. At resolutions of a few (2–4) nanometers it provides unprecedented insights into the molecular organization of cellular landscapes and helps to bridge the divide that hitherto existed between molecular and cellular structural studies.


Electron Dose Conventional Electron Microscopy Optimistic Tilt Cryoelectron Tomography Electron Microscopy Specimen 
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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Max-Planck-Institute of BiochemistryMartinsriedGermany

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