Cryoelectron Tomography or Doing Structural Biology In Situ
Electron tomography enables the three-dimensional visualization of large and stochastically variable structures such as supramolecular assemblies, organelles or even cells. In conjunction with cryogenic techniques electron tomography avoids the artefacts that are notorious to conventional electron microscopy specimen preparation. At resolutions of a few (2–4) nanometers it provides unprecedented insights into the molecular organization of cellular landscapes and helps to bridge the divide that hitherto existed between molecular and cellular structural studies.
KeywordsElectron Dose Conventional Electron Microscopy Optimistic Tilt Cryoelectron Tomography Electron Microscopy Specimen