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Laboratory Methods in Epigenetic Epidemiology

  • Ludovic Barault
  • Rebecca C. Rancourt
Chapter

Abstract

Epidemiology seeks to identify risk factors for disease and to quantify the association between environmental and lifestyle factors and disease frequency in human populations. Epigenetics may provide mechanistic clues on a cellular and molecular level behind epidemiologic links. Combining these two fields along with collecting relevant biospecimens will aid in understanding the benefits and the limitations of genome-wide and gene-specific approaches in decoding the epigenome and its functional relevance. This chapter addresses the importance of the experimental design, optimal preparation of biological samples in population-based studies, provide an overview of the available techniques to analyze DNA methylation, gene expression and histone modification, and highlight possible pitfalls of these approaches.

Keywords

Histone Modification Imprint Gene Nucleosome Occupancy Bisulfite Conversion Bisulfite Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Obstetrics and Gynecology Epidemiology Center, Department of Obstetrics, Gynecology and Reproductive BiologyBrigham and Women’s Hospital, Harvard Medical SchoolBostonUSA

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