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Green Solvents I pp 67–120Cite as

Green Fluids Extraction and Purification of Bioactive Compounds from Natural Materials

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Abstract

This article introduces green solvent extraction and purification of few marker compounds from propolis and rice bran using supercritical carbon dioxide (SC-CO2). The purity (41.2 wt%) of 3,5-diprenyl-4-hydroxycinnamic acid (DHCA) was recovered from propolis using SC-CO2 at 207 bar and 323 K with ethyl acetate (6 wt%) addition. The addition of a normal-phase column adsorption approach was directly employed to obtain purified product containing 95 DHCA by weight. SC-CO2 antisolvent micronization at 200 bar and 328 K generated the submicron particulates containing DHCA (35.2 wt%) from the solution of Brazilian propolis extracts, and the enhancement factor for DHCA concentration reached to 1.61. The DHCA effectively inhibited the growth of human leukemia, colon as well as breast cancer cells, and the human serum low-density lipid oxidation in bioassay. This work also elucidates SC-CO2 extraction of rice bran oil at 300 bar and 313 K from 1.03-kg powdered rice bran. The total yield of oil was 15.7% with a free fatty acid (FFA) content of 3.75%, obtained from 20.5 kg of carbon dioxide. An oil retention efficiency of 82.2% and an FFA removal efficiency of 97.8% were achieved by using SC-CO2 deacidification at 250 bar and 353 K with 2,700 g of carbon dioxide consumed. Besides, the two purest γ-oryzanols (>98 wt%) were isolated by preparative reverse-phase high-performance liquid chromatography (HPLC). Furthermore, the central composite response surface methodology (RSM) was applied to predict the optimal operating conditions and to examine the significance of experimental parameters by a statistic analysis.

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Acknowledgments

The authors gratefully acknowledge funding from the National Science Council of the Republic of China, Taiwan (contract no. NSC96-2628-E005-085-MY2; NSC98-2221-E005-053-MY3; NSC99-2622-B005-CC2), and Taichung Veterans General Hospital and National Chung Hsing University, Taiwan (contract no. TCVGH-NCHU 977603) as well as partial support from the Ministry of Education of the Republic of China, Taiwan, under the ATU plan.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, 402, Taiwan, ROC

    Chao-Rui Chen, Ying-Nong Lee, Chun-Ting Shen, Ling-Ya Wang, Chih-Hung Wang, Jia-Jiuan Wu & Chieh-Ming J. Chang

  2. Chemical Engineering Division, Institute of Nuclear Energy Research, 1000 Wen-Hua Road , Lungtan, Taoyuan, 325, Taiwan, ROC

    Chao-Rui Chen

  3. Department of Biochemistry, China Medical University, 91 Hsueh-Shih Road, Taichung, 404, Taiwan, ROC

    Miau-Rong Lee

  4. Department of Nutrition, China Medical University, 91 Hsueh-Shih Road, Taichung, 404, Taiwan, ROC

    Jia-Jiuan Wu & Hsin-Ling Yang

  5. Education and Research Department, Taichung Veterans General Hospital, 160 Chung-Kang Road, Taichung, 407, Taiwan, ROC

    Shih-Lan Hsu

  6. Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, 123 University Road , Touliu, Yunlin, 640, Taiwan, ROC

    Shih-Ming Lai

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  1. Chao-Rui Chen
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Correspondence to Chieh-Ming J. Chang .

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  1. Faculty of Engineering & Technology, Department of Applied Chemistry, Aligarh Muslim University, Aligarh, 202 002, India

    Ali Mohammad

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Chen, CR. et al. (2012). Green Fluids Extraction and Purification of Bioactive Compounds from Natural Materials. In: Mohammad, A. (eds) Green Solvents I. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1712-1_2

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