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Malignant Brain Tumors: Role of Radioresponsive Gene Therapy

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Book cover Tumors of the Central Nervous system, Volume 3

Part of the book series: Tumors of the Central Nervous System ((TCNS,volume 3))

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Abstract

Patients with malignant gliomas have a very poor prognosis. To explore a novel and more effective approach for the treatment of malignant gliomas, a strategy combining suicide gene therapy and radiation treatment (RT) was designed with insertion of a radio-inducible promoter. The plasmids used in this study included one containing the radio-inducible early growth response gene 1 (Egr-1) promoter, which yielded the best response with fractionated RT. Radio-inducible plasmids that included apoptosis-inducible therapeutic genes caused the apoptosis of glioma cells in response to RT in our in vitro experiments. The radio-inducible plasmids were transfected into subcutaneous glioma cells in nude mice by electroporation in vivo. The plasmids could function in vivo in response to RT and caused a reduction of the tumor volume only in mice that were treated with gene therapies and RT. Our research suggested that apoptosis-inducible gene therapies functioned well together with radio-inducible gene therapies based on the synergistic effects that were observed in combination with RT. Thus, radio-inducible gene therapy may have great potential as a novel treatment because this therapeutic system can be spatially or temporally controlled by exogenous RT and provides specificity and safety due to the limitation of area treated by gene therapies, resulting in decreasing the unpleasant v

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Authors and Affiliations

  1. Department of Neurosurgery, Clinical Medicine, Graduate School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Ibaraki, 305-8565, Japan

    Hideo Tsurushima & Akira Matsumura

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  1. Hideo Tsurushima
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  2. Akira Matsumura
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Correspondence to Hideo Tsurushima .

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Editors and Affiliations

  1. , Kean University, Department of Biological Sciences, Morris Avenue 1000, UNION, 07083, New Jersey, USA

    M.A. Hayat

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Tsurushima, H., Matsumura, A. (2011). Malignant Brain Tumors: Role of Radioresponsive Gene Therapy. In: Hayat, M. (eds) Tumors of the Central Nervous system, Volume 3. Tumors of the Central Nervous System, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1399-4_30

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  • DOI: https://doi.org/10.1007/978-94-007-1399-4_30

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  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-1398-7

  • Online ISBN: 978-94-007-1399-4

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