Skip to main content
SpringerLink
Log in

Ultrasensitive Detection of Non-amplified Genomic DNA

  • Conference paper
  • First Online:
Sensors and Microsystems

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 91))

  • 1251 Accesses

Abstract

The combined use of peptide nucleic acid probes and ultrasensitive nanoparticle-enhanced SPRI detection is shown to allow the ultra-sensitive detection of non-amplified genomic DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Shi L, Perkins RG, Fang H, Tong W (2008) Reproducible and reliable microarray results through quality control: good laboratory proficiency and appropriate data analysis practices are essential. Curr Opin Biotechnol 19:10–18

    Article  Google Scholar 

  2. Grasso G, D’Agata R, Zanoli L, Spoto G (2009) Microfluidic networks for surface plasmon resonance imaging real-time kinetics experiments. Microchem J 93:82–86

    Article  Google Scholar 

  3. Zanoli L, D’Agata R, Spoto G (2008) Surface plasmon-based optical detection of DNA by peptide nucleic acids. Minerva Biotecnologica 20:165–174

    Google Scholar 

  4. D’Agata R, Corradini R, Grasso G, Marchelli R, Spoto G (2008) Ultrasensitive detection of DNA by PNA and nanoparticle-enhanced surface plasmon resonance imaging. ChemBioChem 9:2067–2070

    Article  Google Scholar 

  5. D’Agata R, Grasso G, Spoto G (2008) Real-time binding kinetics monitored with surface plasmon resonance imaging in a diffusion-free environment. Open Spectrosc J 1:1–9

    Article  Google Scholar 

  6. Germini A, Rossi S, Zanetti A, Corradini R, Corrado F, Marchelli R (2005) Development of a peptide nucleic acid array platform for the detection of genetically modified organisms in food. J Agric Food Chem 53:3958–3962

    Article  Google Scholar 

Download references

Acknowledgments

We acknowledge support from MIUR (PRIN 2007 n 2007F9TWKE, FIRB RBRN07BMCT). Dr. M. Bigi and Dr. F. Totsingan are thanked for their assistance.

Author information

Authors and Affiliations

  1. Scuola Superiore di Catania, c/o Dipartimento di Scienze Chimiche, Università di Catania, Catania, Italy

    Laura Maria Zanoli

  2. Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, 95125, Catania, Italy

    Roberta D’Agata & Giuseppe Spoto

  3. Dipartimento di Chimica Organica ed Industriale, Università di Parma, Viale G.P. Usberti 17/a, 43100, Parma, Italy

    Roberto Corradini & Rosangela Marchelli

  4. Neotron S.p.a., Stradello Aggazzotti 104, S. Maria di Mugnano, 41010, Modena, Italy

    Cristina Ferretti & Marcello Gatti

Authors
  1. Laura Maria Zanoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roberta D’Agata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Giuseppe Spoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Roberto Corradini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rosangela Marchelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cristina Ferretti
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marcello Gatti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Giuseppe Spoto .

Editor information

Editors and Affiliations

  1. , Dept. of Industrial Chemistry and Materi, University of Messina, Faculty of Engine, Contrada Di Dio, Messina, 98166, Italy

    Giovanni Neri

  2. , Dept. of Matter Physiscs and Electronic, University of Messina, Faculty of Engine, Contrada Di Dio, Messina, 98166, Italy

    Nicola Donato

  3. Dipto. Ingegneria Elettronica, Università di Roma, Tor Vergata, Via del Politecnico 1, Roma, 00133, Italy

    Arnaldo d'Amico

  4. , Dept. of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, Roma, 00133, Italy

    Corrado Di Natale

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media B.V.

About this paper

Cite this paper

Zanoli, L.M. et al. (2011). Ultrasensitive Detection of Non-amplified Genomic DNA. In: Neri, G., Donato, N., d'Amico, A., Di Natale, C. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 91. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1324-6_79

Download citation

  • DOI: https://doi.org/10.1007/978-94-007-1324-6_79

  • Published:

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-1323-9

  • Online ISBN: 978-94-007-1324-6

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation