Electrochemical and Spectroscopic Characterization of Glucose Oxidase Immobilized in Polyvinyl Alcohol and Applications in Glucose Detection

  • D. Chirizzi
  • M. R. Guascito
  • C. Malitesta
  • E. Mazzotta
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 91)

Abstarct

A simple and novel amperometric biosensor for glucose detection is proposed. It is based on the immobilization of glucose oxidase in a poly(vinyl alcol) matrix drop casted on a platinum electrode surface (Pt/GOx-PVA). The composite material GOx-PVA has been characterized by UV-vis spectroscopy to verify the preservation of enzyme structural integrity and of the enzymatic activity in PVA membrane. X-ray Photoelectron Spectroscopy (XPS) characterization revealed a homogeneous film deposited on Pt whose structure is preserved under operative conditions. Glucose was determined in the absence of a mediator used to transfer electrons between the electrode and the enzyme. Amperometric characterization has been performed at ?400 mV by using pulsed amperometric detection (PAD). Under the selected optimal conditions, the biosensor showed wide dynamic range (0.1–37 mM) yielding a low limit of detection (10 ?M). Biosensor performance was satisfactory also in terms of repeatability, reproducibility and anti-interference ability.

Keywords

Wide Dynamic Range Amperometric Response Glucose Biosensor Pulse Amperometric Detection Glucose Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    O’Neill RD, Lowry JP, Rocchitta G, McMahon CP, Serra PA (2008) Designing sensitive and selective polymer/enzyme composite biosensors for brain monitoring in vivo. Tr Anal Chem 27:78–88CrossRefGoogle Scholar
  2. 2.
    Wang J (2008) Electrochemical glucose biosensors. Chem Rev 108:814–825CrossRefGoogle Scholar
  3. 3.
    Heller A, Feldman B (2008) Electrochemical glucose sensors and their applications in diabetes management. Chem Rev 108:2482–2505CrossRefGoogle Scholar
  4. 4.
    Zhang W, Li J (2004) Third-generation biosensors based on the direct electron transfer of proteins. Anal Sci 20:603–609CrossRefGoogle Scholar
  5. 5.
    Wong F-L, Adbdul-Aziz A (2008) Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase. J Chem Technol Biot 83:41–46CrossRefGoogle Scholar
  6. 6.
    Massey V (2000) The chemical and biological versatility of riboflavin. Biochem Soc Trans 28:283–296CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • D. Chirizzi
    • 1
  • M. R. Guascito
    • 1
  • C. Malitesta
    • 1
  • E. Mazzotta
    • 1
  1. 1.Dipartimento di Scienza dei MaterialiUniversità del SalentoLecceItaly

Personalised recommendations