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Date Palm Biotechnology pp 119–136Cite as

Bioreactors and Automation in Date Palm Micropropagation

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Abstract

Researchers have successfully micropropagated several cultivars of date palm (Phoenix dactylifera L.) using in vitro tissue culture techniques based on somatic embryogenesis and shoot organogenesis. However, major hindrances exist in scale-up including low productivity of cultures, synchronization of embryogenesis, conversion of matured somatic embryos, low multiplication rates of de novo shoots and high production cost of plantlets. Recently, tremendous success has been achieved in automation of micropropagation steps of many plant species using ­liquid culture systems. This achievement constitutes an alternative for resolving all the preceding problems. For example, yield of cotyledonary somatic embryos ­produced in suspension cultures were 17-fold greater than on agar-solidified medium. The transfer of shoot clusters in temporary immersion bioreactor clearly improved the yield of regenerated shoots 5.5-fold in comparison with that regenerated on agar-solidified medium. The aim of this chapter is to critically outline the potential of liquid culture systems (suspension culture and temporary immersion system, TIB) to large scale and automation of date palm micropropagation. The principles, advantages and disadvantages of these methods are also described.

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Authors and Affiliations

  1. Laboratory of Molecular Genetics, Immunology & Biotechnology, Faculty of Sciences of Tunis, 2092, El Manar, Tunis, Tunisia

    A. Othmani & M. Trifi

  2. Laboratory of Plant Molecular Physiology, Biotechnology Center of Borj Cédria, 901, Hammam-Lif, Tunisia

    R. Mzid

  3. Laboratory of In Vitro Culture, Regional Center of Oases Agriculture Researches, 2260, Degache, Tunisia

    C. Bayoudh

  4. Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, 3018, Sfax, Tunisia

    N. Drira

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  1. A. Othmani
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  2. R. Mzid
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  3. C. Bayoudh
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  4. M. Trifi
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  5. N. Drira
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Correspondence to A. Othmani .

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Editors and Affiliations

  1. , Department of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. , Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, 31982, Saudi Arabia

    Jameel M. Al-Khayri

  3. Middlebrook Ave 3726, Cincinnati, 45208, Ohio, USA

    Dennis V. Johnson

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Othmani, A., Mzid, R., Bayoudh, C., Trifi, M., Drira, N. (2011). Bioreactors and Automation in Date Palm Micropropagation. In: Jain, S., Al-Khayri, J., Johnson, D. (eds) Date Palm Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1318-5_7

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