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Piezoelectric Sensing for Sensitive Detection of DNA

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Detection of Non-Amplified Genomic DNA

Part of the book series: Soft and Biological Matter ((SOBIMA))

Abstract

Piezoelectric sensing has been widely applied for affinity sensing, and recently sensitive DNA detection has been reported in different matrices for different analytes (i.e. target sequences). In this chapter, the detection principle and the approaches used in DNA-based sensing with focus on detection of microsatellite DNA, present in high number of copy as well as target sequence detection of genes present in one or few copy number per haploid genome will be presented and discussed. Particular attention will be devoted to the pre-analytical steps which may influence the sensor response to the target analyte such as genomic DNA fragmentation and denaturation. Comparison between immobilization chemistries is also presented. In particular, finding in microsatellite detection with both biotinylated and thiolated probes is reported and discussed.

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Authors and Affiliations

  1. Dipartimento di Chimica “Ugo Schiff”, Università di Firenze, Via della Lastruccia 3, 50019, Sesto F.no (FI) Florence, Italy

    Maria Minunni

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  1. Maria Minunni
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Correspondence to Maria Minunni .

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  1. , Department of Chemical Science, University of Catania, Viale Andrea Doria 6, Catania, 95125, Italy

    Giuseppe Spoto

  2. , Faculty of Science, University of Parma, Parma, 43100, Italy

    Roberto Corradini

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Minunni, M. (2012). Piezoelectric Sensing for Sensitive Detection of DNA. In: Spoto, G., Corradini, R. (eds) Detection of Non-Amplified Genomic DNA. Soft and Biological Matter. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1226-3_8

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