Advertisement

Carbon Isotope Effects as a Tool to Study Photosynthesis

  • Alexander A. Ivlev
Conference paper
Part of the NATO Science Series book series (NAII, volume 129)

Abstract

Previously it was shown [1] that metabolic carbon isotope effects (13C/12C) are a useful tool to investigate temporal organization of cell processes. The analysis of carbon isotopic composition of metabolites and their isotopic patterns proved the existence of a certain sequence of metabolic events in glycolytic chain of heterotrophic and photosynthesizing organisms. This finding agrees with the idea [2] that the processes in glycolytic chain oscillate. In present lecture we’ll try to apply the same approach to investigate photosynthesis organization.

Keywords

Carbon Isotope Carbon Flux Isotope Fractionation Isotope Effect Carbon Isotope Composition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ivlev, A.A. (1992) Distribution of carbon isotopes (13C/12C) in the cell and temporal organization of cellular processes, Bioflzika (russ.) 36, 1078–1087.Google Scholar
  2. 2.
    Sel’kov, Ye. Ye. (1978) Temporal organization of energy metabolism and cell clock, M.N.Kondrashova (ed.) Regulation of Energy Exchange and the Physiological State of the Organism (russ.) Nauka, Moscow, pp. 15-32.Google Scholar
  3. 3.
    Craig, H. (1957) Isotopic standards for carbon and oxygen and corrections for mass-spectrometric analysis of carbon dioxide,. Geochim. et Cosmochim.Acta 12, 133-149.Google Scholar
  4. 4.
    Degens, E.T. (1969) Biogeochemistry of stable carbon isotopes, G.Englinton and M.T.J.Murphy, (eds.) Organic geochemistry. Method and results. Springer-Verlag, Berlin, Heidelberg, New York, pp.304-330.Google Scholar
  5. 5.
    Abelson, P.H., and Hoering, T.C. (1961) Carbon isotope fractionation in formation of amino acids by photosynthetic organisms, Proc. Natl. Acad.Sci. USA 47, 623–631.CrossRefGoogle Scholar
  6. 6.
    Smith, B.N, and Epstein, S. 1971. Two categories of 13C/12C ratios for higher plants,. Plant Physiol. 47, 380-384.Google Scholar
  7. 7.
    Bender, M.M. Rouhani, I., Vines, H.M., and Black, C.C. (1973) 13C/12C ratio changes in Crassulecean acid metabolism,. Plant Physiol. 52, 427-430.Google Scholar
  8. 8.
    Vogel, J.C. (1993) Variability of carbon isotope fractionation, Ehleringer J.R., Hall A.E., Farquhar G.D (eds.). San Diego, Boston, Stable isotopes and plant carbon-water relations. Academic Press, Inc. NewYork, London, Tokyo pp.29-45.Google Scholar
  9. 9.
    Park, R., and Epstein, S. (1960) Carbon isotope fractionation during photosynthesis. Geochim. Cosmochim. Acta 21, 110-126.Google Scholar
  10. 10.
    Park, R., and Epstein, S. (1961) Metabolic fractionation of 13C/12C in plants, Plant Physiol. 36, 133–139.CrossRefGoogle Scholar
  11. 11.
    Whelan,T.W., Sackett,W.M, and Benedict CR. (1973) Enzymatic fractionation of carbon isotopes by phosphoenolpyruvate carboxylase from C4-plants. Plant Physiol 51, 1051-1054.Google Scholar
  12. 12.
    Reibach,M.R., and Benedict, CR. (1974) Fractionation of stable carbon isotopes by phosphoenolpyruvate carboxylase from C4-piants. Plant Physiol. 59, 564–568.CrossRefGoogle Scholar
  13. 13.
    Ivlev, A.A., and Knyazev,. D.A. (1986) The analysis of carbon isotope effects in enzymic RyBP carboxylation. Izvestiya Timiryazevskoy seVsko-khozyaystvehhoy akademii (russ.). N 2, 175-180.Google Scholar
  14. 14.
    Estep, M.L.F., Tabita, F.R., Parker, P.L., and Baalen, Ch.V. (1978) Carbon isotope fractionation by ribulose-1, 5-biphosphate carboxylase from various organisms. Plant Physiol. 61, 680-687.Google Scholar
  15. 15.
    Farquhar, G.D., O’Leary, M.H., and Berry, J.A. (1982) On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Aust. J. Plant Physiol. 9, 121–137.CrossRefGoogle Scholar
  16. 16.
    Piesker M. (1986) Models of carbon metabolism in C3-C4 intermediate plants as applied to the evolution of C4 photosynthesis. Plant Cell and Environment 9, 627–635.CrossRefGoogle Scholar
  17. 17.
    Vogel J.C. Variability of carbon isotope fractionation 1993. Stable isotopes and plant carbon-water relations. J.R.Ehleringer, A.E.Hall, and G.D. Farquhar (eds.) Academic Press, Inc. San Diego, Boston, N.Y., London, Tokyo, pp.29-45.Google Scholar
  18. 18.
    Jillon, J.S., and Griffiths, H. (1997) The Influence of (Photo)Respiration on carbon isotope discrimination in plants. Plant, Cell and Environment 20, 1217–1230.CrossRefGoogle Scholar
  19. 19.
    Laisk, A. Kh. 1977. Kinetics of photosynthesis and photorespiration in C-3 plants (russ.) Nauka. Moscow.Google Scholar
  20. 20.
    Calvin,M., Parker,P.L., and Fry,B. (1980) Carbon-13/Carbon-12 ratios in seagrasses. Aquat. Botany 9, 237-249.Google Scholar
  21. 21.
    Benedict, CR., Wong, W.W., and Wong, J.H. (1980) Fractionation of the stable isotopes of inorganic carbon by seagrasses. Plant Physiology 65, 512–517.CrossRefGoogle Scholar
  22. 22.
    Ivlev, A.A., (1992) Carbon isotope effects and a coupled mechanism of photosynthesis and photorespiration. Sov.Plant Physiol. 39, 825–835.Google Scholar
  23. 23.
    Sanadze, G.A., Black, C.C., Tevzadze, I.T., and Tarkhnishvili, G.M. (1978) A change in the 13CO2/12CO2 isotope ratio during photosynthesis by C3 and C4-plants. Fiziol. Rast, (russ.) 25, 171-172.Google Scholar
  24. 24.
    Voznesenskii, V.L., Glagoleva, T.A, Zubkova, E.K., Mamushina, N.S., Filippova, L.A., and Chulanovskaya, M.V. (1982) Metabolism of 14C in Chlorella during prolonged cultivation in the presence of 14CO2. Fiziol. Rast, (russ.) 29, 564–571.Google Scholar
  25. 25.
    Ivanov, M.V., Zyakun, A.M., Gogotova, G.I., Bondar’, V.A. (1978) Fractionation of carbon isotopes by photosinthesising bacteria, grown on bicarbonate enriched in carbon-13. Dokl. Akad. Nauk SSSR (russ.) 242, 1417–1420.Google Scholar
  26. 26.
    Ivlev, A.A. (1993) On the flows of “light” and “heavy” carbon during photosynthesis and photorespiration coupling. RussJ.Plant Physiol. 40, 871–878.Google Scholar
  27. 27.
    Ivlev, A.A., Kalinkina, L.G. (2001) Experimental evidence for the isotope effect in photorespiration. RussJ.Plant. Physiology. 48, 400–412.CrossRefGoogle Scholar
  28. 28.
    Ivlev, A.A. (2001) Carbon isotope effects (13C / 12C ) in biological systems. Separation Science and Technology 86, 1815–1910.Google Scholar
  29. 29.
    Semenenko, V.E. (1964) The investigation of the mechanism of the processes determining the peculiarities of CO2 absorbtion kinetics at the beginning of inductional phase of photosynthesis. Fiziologiya rastenii (russ. )11, 216–231.Google Scholar
  30. 30.
    Yordan, F., Kuo, D. J., and Monse, E.U. (1978) Carbon kinetic isotope effects on pyruvate decarboxylation catalyzed by yeast pyruvate decarboxylase and models. J. Am.Chem.Soc. 100, 2872–2878.CrossRefGoogle Scholar
  31. 31.
    Seltzer, S., Hamilton, G.A., and Westheimer, F.H. (1979) Isotope effect in the enzymatic decarboxylation of oxaloacetic acid. J. Am.Chem.Soc. 81, 4018–4021.CrossRefGoogle Scholar
  32. 32.
    O’Leary, M.H., Piazz, G.G. (1981). Medium effect in enzyme catalyzed decarboxylation. Biochemistry 20, 2743–2748.CrossRefGoogle Scholar
  33. 33.
    Ivlev, A.A., Bykova, N.V., and Igamberdiev, A.U. (1996) Fractionation of carbon (13C/12C) isotopes in glycine decarboxylase reaction. FEBS Letters 386, 174–176.CrossRefGoogle Scholar
  34. 34.
    Ivlev, A.A., Igamberdiev, A.U., Threlkeld, Ch., and Bykova, N.V. (1999) Carbon isotope effects in the glycine decarboxylase reaction in vitro on mitochondria from pea and spinach. Russ.J. Plant Physiol. 46, 653-660.Google Scholar
  35. 35.
    Igamberdiev, A.U., Ivlev, A.A., Bykova, N.V., Threlkeld, Ch., Lea, P.J., and Gardestrom, P. (2001) Decarboxylation of glycine contributes to carbon isotope fractionation in photosythetic organisms. Photosynthesis Research. 67, 177–184.CrossRefGoogle Scholar
  36. 36.
    Lenne, C, Neuburger, M., Douce, R. (1993) Effect of high physiological temperatures on NAD+ content of green leaf mitochondria-apparent inhibition of glycine oxidation. Plant Physiol. 102, 1157–1162.Google Scholar
  37. 37.
    Oliver D.J. (1994) The glycine decarboxylase complex from plant mitochondria. Annu.Rev.Plant Physiol.Plant Mol.Biol. 45, 323–337.CrossRefGoogle Scholar
  38. 38.
    Jillon, J.S., and Griffiths, H. (1997) The Influence of (Photo)Respiration on Carbon Isotope Discrimination in Plants Plant, Cell and Environment 20, 1217–1230.CrossRefGoogle Scholar
  39. 39.
    Lin, G.H., and Ehleringer, J.R. (1997) Carbon isotope fractionation doesn’t occur during dark photorespiration in C3 and C4. Plant Physiol. 114, 391–394.Google Scholar
  40. 40.
    Borland, A.M., Griffiths, H., Broadmeadow, M.S., Fordham, M.C., and Maxwell, C. (1994) Carbon isotope composition of biochemical fractions and the regulation of carbon balance in leaves of the C3-Crassulenean acid metabolism intermediate Clusia minor L. growing in Trinidad. Plant Physiol. 105, 493-501Google Scholar
  41. 41.
    Schmidt, H.-L., Kexel, K, Butzenlechner, M., Schwarz, S., Gleixner, G, Thimet, S., Werner, R.A. and Gensler, M. (1995) 2. Non-statistical isotope distribution in natural compounds: mirror of their biosynthesis and key for their origin assignment, in E. Wada, T. Yoneyama, M Minagawa., T. Ando, and B.D. Fry (eds.) Stable Isotopes in the Biosphere. University Press Kyoto: Kyoto,, pp. 17-35.Google Scholar
  42. 42.
    Rivera, E.R., and Smith, B.N. (1979) Crystal morphology and 13Carbon/12Carbon composition of solid oxalate in Cacti. Plant Physiol. 64, 966–970.CrossRefGoogle Scholar
  43. 43.
    Raven, J.A., Griffiths, H., Glidewell, S.M., Preston, T. (1982) The mechanism oxalate biosynthesis in higher plants: investigations with the stable isotopes oxygen-18 and carbon-13. Proc.R.Soc. (London). Ser.B, 216, 87–101.CrossRefGoogle Scholar
  44. 44.
    Igamberdiev A.U. (1988) Photorespiration and biochemical evolution of plants. Uspekhi Sovr. Biologii (russ.) 105, 488–504Google Scholar
  45. 45.
    Igamberdiev A.U. (1991) Peroxisomal oxidation in plants. Fiziologiya rastenii (russ.) 38, 773–785.Google Scholar
  46. 46.
    Kalinkina, L.G., and Udel’nova, T.M. (1990) Effect of photorespiration on the stable carbon isotope fractionation in marine Chlorella. Fiziol. Rast, (russ.) 37, 96–104.Google Scholar
  47. 47.
    Kalinkina, L.G., and Naumova, T.S. (1993) The role of photorespiration in free proline accumulation in Chlorella stigmatophora cells under salinity. Russ.J.Plant Physiol. 40, 577–583.Google Scholar
  48. 48.
    Metun, J. (1963) Biosynthesis of amino acids, in P. Bernfeld (ed.) Biogenesis of Natural Compounds. Pergamon Press. Oxford, pp.9-33.Google Scholar
  49. 49.
    Strickland, K.P. (1963) Biogenesis of lipids, in P. Bernfeld (ed.) Biogenesis of Natural Compounds. Pergamon Press. Oxford, pp.82-131.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  • Alexander A. Ivlev
    • 1
  1. 1.Moscow Agriculture Academy of K.A. TimiryazevMoscowRussia

Personalised recommendations