Skip to main content
Book cover

Bacterial Adhesion pp 257–269Cite as

Electron Microscopy Techniques to Study Bacterial Adhesion

Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 715)

Abstract

Since its introduction 70 years ago electron microscopy has become an invaluable tool for microbiology and the study of bacterial interaction. Technological development over the past decades has enabled researchers to resolve smaller and smaller details in bacterial samples, while new preparation techniques like cryo preparation now allow to investigate bacteria even closer to their natural state. In this chapter we give a brief overview of electron microscopy techniques suitable for the investigation of bacterial adhesion at molecular as well as cellular level and a short outlook on future technologies relevant to the field.

Keywords

  • Bacterial Adhesion
  • Phase Contrast Imaging
  • Chemical Fixation
  • Bacterial Cell Suspension
  • Scanning Electron Microscopy Sample

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-94-007-0940-9_16
  • Chapter length: 13 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   179.00
Price excludes VAT (USA)
  • ISBN: 978-94-007-0940-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   229.99
Price excludes VAT (USA)
Hardcover Book
USD   299.99
Price excludes VAT (USA)
Learn about institutional subscriptions
Fig. 16.1
Fig. 16.2
Fig. 16.3
Fig. 16.4
Fig. 16.5
Fig. 16.6
Fig. 16.7
Fig. 16.8

References

  • Bayer ME, Carlemalm E, Kellenberger E (1985) Capsule of Escherichia coli K29: ultrastructural preservation and immunoelectron microscopy. J Bacteriol 162:985–991

    PubMed  CAS  Google Scholar 

  • Beutin L, Strauch E, Zimmermann S, Kaulfuss S, Schaudinn C, Männel A, Gelderblom HR (2005) Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48. BMC Microbiol 5:4

    PubMed  CrossRef  Google Scholar 

  • Brenner S, Horne RW (1959) A negative staining method for high resolution eclectron microscopy of viruses. Biochim Biophys Acta 14:103–110

    CrossRef  Google Scholar 

  • Danev R, Nagayama K (2010) Phase plates for transmission electron microscopy. Methods Enzymol 481:343–369

    PubMed  CrossRef  Google Scholar 

  • Dehio C, Meyer M, Berger J, Schwarz H, Lanz C (1997) Interaction of Bartonella henselae with endothelial cells results in bacterial accumulation and aggregation on the cell surface and the subsequent engulfment and internalisation of the bacterial aggregate by a well-defined structure, the invasome. J Cell Sci 110:2141–2154

    PubMed  CAS  Google Scholar 

  • Dubochet J, Adrian M, Chang JJ, Homo JC, Lepault J, McDowall AW, Schultz P (1988) Cryo-electron microscopy of vitrified specimens. Q Rev Biophys 21:129–228

    PubMed  CrossRef  CAS  Google Scholar 

  • Frank J (2006) Three-dimensional electron microscopy of macromolecular assemblies: visualization of biological molecules in their native state. Oxford University Press, Oxford

    CrossRef  Google Scholar 

  • Geerts WJC, Humbel BM, Verkleij AJ (2008) 3-D electron tomography of cells and organelles. In: Cavalier A, Spehner D, Humbel BM (eds) Handbook of cryo-preparation methods for electron microscopy, chapter 21. CRC Press, Boca Raton, FL, pp 537–565

    Google Scholar 

  • Gonen T, Cheng Y, Sliz P, Hiroaki Y, Fujiyoshi Y, Harrison SC, Walz T (2005) Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438:633–638

    PubMed  CrossRef  CAS  Google Scholar 

  • Griffiths G (1993) Fine structure immuno-cytochemistry. Springer, Berlin

    Google Scholar 

  • Haider M, Müller H, Uhlemann S, Zach J, Loebau U, Hoeschen R (2008) Prerequisites for a Cc/Cs corrected ultrahigh resolution TEM. Ultramicroscopy 108:167–178

    PubMed  CrossRef  CAS  Google Scholar 

  • Hayat MA (1981) Fixation for biological electron microscopy. Academic, New York, NY

    Google Scholar 

  • Hayat MA (2000) Principles and techniques of electron microscopy. Biological applications. Cambridge University Press, Cambridge

    Google Scholar 

  • Hohenberg H, Mannweiler K, Müller M (1994) High-pressure freezing of cell suspensions in cellulose capillary tubes. J Microsc 175:34–43

    PubMed  CAS  Google Scholar 

  • Hosogi N, Shigematsu H, Terashima H, Homma M, Nagayama K (2011) Zernike phase contrast cryo-electron tomography of sodium-driven flagellar hook-basal bodies from Vibrio alginolyticus. J Struct Biol 173:67–76

    PubMed  CrossRef  CAS  Google Scholar 

  • Knutton S (1995) Electron microscopical methods in adhesion. Meth Enzymol 253:145–158

    PubMed  CrossRef  CAS  Google Scholar 

  • Koster AJ, Grimm R, Typke D, Hegerl R, Stoschek A, Walz J, Baumeister W (1997) Perspectives of molecular and cellular electron tomography. J Struct Biol 120:276–308

    PubMed  CrossRef  CAS  Google Scholar 

  • Larabell CA, Nugent KA (2010) Imaging cellular architecture with X-rays. Curr Opin Struct Biol 20:623-631

    PubMed  CrossRef  CAS  Google Scholar 

  • McDermott G, Le Gros MA, Knoechel CG, Uchida M, Larabell CA (2009) Soft X-ray tomography and cryogenic light microscopy: the cool combination in cellular imaging. Trends Cell Biol 19:587–595

    PubMed  CrossRef  CAS  Google Scholar 

  • Neu TR, Poralla K (1988) An amphiphilic polysaccharide from an adhesive Rhodococcus strain. FEMS Microbiol Lett 49:389–392

    CrossRef  CAS  Google Scholar 

  • Piekarski G, Ruska H (1939) Übermikroskopische Darstellung von Bakteriengeisseln. Klin Wschr 18:383–386

    Google Scholar 

  • Pilhofer M, Ladinski MS, McDowall AW, Jensen GJ (2010) Bacterial TEM: new insights from cryo-microscopy. Meth Cell Biol 96:21–45

    CrossRef  Google Scholar 

  • Schenk AD, Hite RK, Engel A, Fujiyoshi Y, Walz T (2010) Electron crystallography and aquaporins. Methods Enzymol 483:91–119

    PubMed  CrossRef  CAS  Google Scholar 

  • Schwarz H, Humbel BM (2008) Correlative light and electron microscopy. In: Cavalier A, Spehner D, Humbel BM (eds) Handbook of cryo-preparation methods for electron microscopy, chapter 21. CRC Press, Boca Raton, FL, pp 537–565

    Google Scholar 

  • Szczesny P, Linke D, Ursinus A, Bär K, Schwarz H, Riess TM, Kempf VA, Lupas AN, Martin J, Zeth K (2008) Structure of the head of the Bartonella adhesin BadA. PLoS Pathog 4(8):e1000119

    PubMed  CrossRef  Google Scholar 

  • Tokuyasu KT (1973) A technique for ultracryotomy of cell suspensions and tissues. J Cell Biol 57:551–565

    PubMed  CrossRef  CAS  Google Scholar 

  • Tokuyasu KT (1997) Immunocytochemistry on Ultrathin Cryosections. In: Spector DL, Goldman RD, Leinwand LA (eds) Cells – A Laboratory Manual, Chapter 131, Cold Spring Harbor Laboratory Press, pp 1–27

    Google Scholar 

  • von Ardenne M (1940) Elektronen-Übermikroskopie. Springer, Berlin

    Google Scholar 

  • von Borries B, Ruska E, Ruska H (1938) Bakterien und Virus in übermikroskopischer Aufnahme. Klin Wschr 17:921–925

    CrossRef  Google Scholar 

  • Walther P, Müller M (1999) Biological ultrastructure as revealed by high resolution cyro-SEM by block faces after cryosectioning. J Microsc 196:279–287

    PubMed  CrossRef  CAS  Google Scholar 

  • Walther P, Wehrli E, Hermann R, Müller M (1995) Double layer coating for high resolution low temperature SEM. J Microsc 179:229–237

    PubMed  CAS  Google Scholar 

  • Yamaguchi M, Danev R, Nishiyama K, Sugawara K, Nagayama K (2008) Zernike phase contrast electron microscopy of ice-embedded influenza A virus. J Struct Biol 162:271–276

    PubMed  CrossRef  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max Planck Institute for Developmental Biology, Tübingen, Germany

    Iwan Grin & Heinz Schwarz

  2. Department of Protein Evolution, Max Planck Institute for Developmental Biology, 72076, Tübingen, Germany

    Dirk Linke

Authors
  1. Iwan Grin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Heinz Schwarz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dirk Linke
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dirk Linke .

Editor information

Editors and Affiliations

  1. , Department I, Protein Evolution, Max-Planck Institute for Developmental B, Spemannstr. 35, Tübingen, 72076, Germany

    Dirk Linke

  2. , Institute of Biotechnology, University of Helsinki, Viikinkaari 1, Helsinki, FIN-00014, Finland

    Adrian Goldman

Rights and permissions

Reprints and Permissions

Copyright information

© 2011 Springer Science+Business Media B.V.

About this chapter

Cite this chapter

Grin, I., Schwarz, H., Linke, D. (2011). Electron Microscopy Techniques to Study Bacterial Adhesion. In: Linke, D., Goldman, A. (eds) Bacterial Adhesion. Advances in Experimental Medicine and Biology, vol 715. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0940-9_16

Download citation