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Crystallography and Electron Microscopy of Chaperone/Usher Pilus Systems

Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 715)

Abstract

Among bacteria, the chaperone-usher (CU) pathway is a widespread conserved assembly and translocation system for adhesive protein fibres, called pili or fimbriae. Pili are large linear polymers that protrude from the outer bacterial surface and consist of several subunits. Pili contain adhesin proteins at the tip that are used by pathogenic bacteria to mediate attachment to host cells and initiate infections. Well studied examples of CU pili are P and type 1 pili of uropathogenic Escherichia coli (UPEC), which are responsible for kidney and bladder infections, respectively. Upon secretion into the periplasm, pilus subunits are stabilized by periplasmic chaperones and the resulting chaperone:subunit complexes are guided to the usher located in the outer membrane. The usher catalyzes the ordered assembly of pilus subunits while releasing the chaperones and translocating the growing pilus stepwise to the outer surface. Here we review the structural biology of the chaperone-usher pathway that has helped to understand the mechanisms by which biogenesis of an important class of bacterial organelles occurs.

Keywords

  • Pilus Assembly
  • Periplasmic Chaperone
  • Pilus Biogenesis
  • Pilus Subunit
  • Plug Domain

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Correspondence to Gabriel Waksman .

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Geibel, S., Waksman, G. (2011). Crystallography and Electron Microscopy of Chaperone/Usher Pilus Systems. In: Linke, D., Goldman, A. (eds) Bacterial Adhesion. Advances in Experimental Medicine and Biology, vol 715. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0940-9_10

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