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Cell Lines in Four Weeks with the CEMAX® System

  • Benedikt Greulich
  • Karlheinz Landauer
  • Andreas Herrmann
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 5)

Abstract

Cell line development up to now is a time consuming and laborious process, since it is based on statistical integration of the transgene encoding the protein of interest. Thus high producer clones have to be identified in an intensive screening process. Usually the productivity of these clones is further improved in one or more gene amplification steps although this could reduce genetic stability. This study presents the CEMAX® system, a novel mammalian expression system for the generation of CHO K1 derived production cell lines for biopharmaceuticals and its use for the production of highly glycosylated proteins. Stable high producers are available within four weeks after transfection and with less effort compared to conventional methods. This was achieved by adopting site-specific integration of the gene of interest.

Keywords

Selection Marker Spinner Flask Exchange Vector Intensive Screening Mammalian Expression System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors like to thank Silke Schindler for technical assistance.

References

  1. Choulika, A., et al., Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae. Mol Cell Biol, 1995. 15(4): 1968–1973.PubMedGoogle Scholar
  2. Sambrook, J. and D.W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed. 2001, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
  3. Zahn, S., et al., Highly Efficient Serum-Free Production of Biopharmaceuticals in CHO-Cells, in Cell Technology for Cell Products; Proceedings of the 19th ESACT Meeting, Harrogate, UK. 2007. pp. 765–767.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Benedikt Greulich
    • 1
  • Karlheinz Landauer
    • 1
  • Andreas Herrmann
    • 1
  1. 1.Celonic AGBaselSwitzerland

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