Mammalian cells for production of biologics used in industry are immortal and so they continue multiplying even after they reach required cell number. This excess proliferation consumes nutrients in the medium uselessly, suggesting that appropriate proliferation control decreases consumption of medium. In this study, we aimed to reveal how intracellular factors could participate in growth control, and for the purpose, one murine hybridoma cell line and its serum-free sub-line were investigated as model cells. They opposite response to interleukin-6; in the presence of IL-6, proliferation of original line is accelerated, while that of adapted line is decelerated. Comparison of intracellular factors in both cell lines was done in order to get insight into the control of cell proliferation.
Trypan Blue Mitogenic Effect STAT3 Inhibitor Opposite Response Viable Cell Density
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This study was partly supported by the grant of Alumni Association of Engineering, Fukui University.
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