Simulation and Optimization of Essential Amino Acids in Dynamic Mammalian Cell Culture
Optimization in mammalian cell culture systems is currently exclusively experimental, which is an expensive and time-consuming process. Mathematical models have become established practice in many process industries. Herein, a systematic framework has been developed that allows the simulation and off-line optimization of fed-batch monoclonal antibody-producing hybridoma cell cultures. The model describes major cellular functions as well as the uptake of amino acids and how it affects growth and productivity. The model-based optimization approach was able to provide an estimation of the optimal dynamic profile of essential amino acids in order to improve the IFNγ yield.
KeywordsEssential Amino Acid Dynamic Optimization Mammalian Cell Culture Global Sensitivity Analysis Model Amino Acid
We would like to thank Dr. Sergei Kucherenko for his help with the GSA analysis and the Hutchinson-Whampoa BBSRC Dorothy Hodgkin Postgraduate Award for supporting this research.