Study of the Effect of High pH and Alkali Addition in a Cultivation of Chinese Hamster Ovary Cell
This work aimed at studying the impact of alkali addition in a Chinese Hamster Ovary cell culture. Two phenomena were studied, the kinetic rate of direct cell death in presence of high pH and the effect of transitory single contact of high pH on cell viability and growth. Contact with pH 11 or 10 did not provoke immediate cell lysis. The cells survived several minutes to such conditions. Contact with pH 11 during 2 minutes, with pH 10 during 5 min, with pH 9 during 5 minutes or 10 min did not affect the viability. In these conditions, the growth was not affected except after 5 min contact at pH 10 or 10 min contact at pH 9 for which the growth was slowed down the first day only. As expected, NaOH addition affected the cells more than Na2CO3 addition. This was due to a higher pH but could be even observed at the same pH (10).
KeywordsChinese Hamster Ovary Cell High Osmolarity Cell Cultivation Process Alkali Addition Osmolarity Measurement
We acknowledge The Swedish Governmental Agency for Innovation Systems (VINNOVA), which supported this work. We thank IMED, Ab,Sweden, for their kind permission of using their research cell line. Many thanks to Irvine Scientific, USA, for the sponsor of medium. Finally thank you to Helene Sundström for her extensive support in the lab and to Kaj Kauppi for his help with microscope photography.