Abstract
Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development. In this report, we demonstrate enhanced, high-level monoclonal antibody (mAb) titres of 136 mg/L with CHO cells using the episomal-based transient expression system, Epi-CHO. These high titres were achieved by implementing an optimised transfection protocol and through screening of a variety of chemically defined and serum-free media for their ability to support elevated and prolonged viable cell densities post-transfection, and in turn, improve recombinant protein yields. The Epi-CHO system allows for scalable and rapid production of CHO cell-derived recombinant proteins in serum-free conditions.
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Acknowledgements
The authors would like to thank Karen Hughes and the NCRIS Biologics Facility at the AIBN and Robert Simpson from the Real-time PCR Facility at The University of Queensland.
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Codamo, J., Munro, T.P., Hughes, B.S., Song, M., Gray, P.P. (2012). An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media. In: Jenkins, N., Barron, N., Alves, P. (eds) Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. ESACT Proceedings, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0884-6_3
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DOI: https://doi.org/10.1007/978-94-007-0884-6_3
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