An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media
Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development. In this report, we demonstrate enhanced, high-level monoclonal antibody (mAb) titres of 136 mg/L with CHO cells using the episomal-based transient expression system, Epi-CHO. These high titres were achieved by implementing an optimised transfection protocol and through screening of a variety of chemically defined and serum-free media for their ability to support elevated and prolonged viable cell densities post-transfection, and in turn, improve recombinant protein yields. The Epi-CHO system allows for scalable and rapid production of CHO cell-derived recombinant proteins in serum-free conditions.
KeywordsHeavy Chain Viable Cell Density Transient Expression System Recombinant Protein Yield Optimise Transfection
The authors would like to thank Karen Hughes and the NCRIS Biologics Facility at the AIBN and Robert Simpson from the Real-time PCR Facility at The University of Queensland.