Platform Technology for Viral Vaccine Production: Comparison Between Attached and Suspension Vero Cells
Vero cells can be used as host for the production of many different types of viruses. The standard Vero culturing process consists of growing Vero cells adherent to microcarriers. Due to surface limitation the cells will have to be transferred to new microcarriers regularly. This subculturing of the cells, especially at large scale, is a complicated technique. This drawback can be overcome by using single cell suspension cultures. Vero cells growing in single cell suspension (sVero) were compared to adherent growing Vero cells (aVero) for their capability of producing polio virus. Using flow cytometry it was determined that sVero cells contained the polio virus receptor and were able to produce polio virus. These results indicate that sVero cells may be an ideal candidate for a platform based viral vaccine production approach. The combination of sVero cells with the flexibility of disposable bioreactors completes their suitability for these purposes.
KeywordsVero Cell Single Cell Suspension Stir Tank Reactor Virus Culture Spinner Flask
We thank Guillermina Forno from Zelltek S.A. and Marina Etcheverrigaray from the Universidad Nacional del Litoral, Argentina for providing the sVero cells.
- Kistner O, Howard MK, Spruth M, Wodal W, Bruhl P, Gerencer M, Crowe BA, Savidis-Dacho H, Livey I, Reiter M, Mayerhofer I, Tauer C, Grillberger L, Mundt W, Falkner FG, Barrett PN. 2007. Cell culture (Vero) derived whole virus (H5N1) vaccine based on wild-type virus strain induces cross-protective immune responses. Vaccine 25:6028–6036.PubMedCrossRefGoogle Scholar
- World Health Organization. 1998. Requirements for the use of animal cells as in vitro substrates for the production of biologicals.WHO Technical Report Series. pp 20–53.Google Scholar