Poxvirus Production on Chicken Embryo Fibroblasts in iCELLis™ Disposable Fixed-Bed Bioreactor
Most veterinary vaccine and some gene therapy companies manufacture products on primary chicken embryo fibroblasts (CEF) extracted from incubated eggs. Productions are carried out in uncontrolled TF, roller bottles or multitray systems.
The drawbacks are the following non-controlled conditions (pH, DO, etc). – large number of open aseptic operations – large space requirements – not always linear scale-up.
Here we present a case study: the culture of CEF and the multiplication of Modified Vaccinia Ankara virus (MVA) in iCELLisTM fixed-bed bioreactors.
Principle of the iCELLisTM fixed-bed technology – scale reduction – is explained in the Table 1 below. The surface available to anchorage-dependent cells is larger in iCELLis than in static systems.