Poxvirus Production on Chicken Embryo Fibroblasts in iCELLis™ Disposable Fixed-Bed Bioreactor

  • Nicolas Havelange
  • Martine Marigliano
  • Martine Sainte-Marie
  • Fabien Debras
  • Nadia Tazir
  • José Castillo
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 5)

Abstract

Most veterinary vaccine and some gene therapy companies manufacture products on primary chicken embryo fibroblasts (CEF) extracted from incubated eggs. Productions are carried out in uncontrolled TF, roller bottles or multitray systems.

The drawbacks are the following non-controlled conditions (pH, DO, etc). – large number of open aseptic operations – large space requirements – not always linear scale-up.

Here we present a case study: the culture of CEF and the multiplication of Modified Vaccinia Ankara virus (MVA) in iCELLisTM fixed-bed bioreactors.

Principle of the iCELLisTM fixed-bed technology – scale reduction – is explained in the Table 1 below. The surface available to anchorage-dependent cells is larger in iCELLis than in static systems.

Keywords

BHK21 Cell Chicken Embryo Fibroblast Scale Reduction Roller Bottle Modify Vaccinia Ankara Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Nicolas Havelange
    • 1
  • Martine Marigliano
    • 2
  • Martine Sainte-Marie
    • 2
  • Fabien Debras
    • 3
  • Nadia Tazir
    • 3
  • José Castillo
    • 1
  1. 1.ATMI LifeSciences/ArtelisBrusselsBelgium
  2. 2.TransgeneStrasbourgFrance
  3. 3.ArtelisBrusselsBelgium

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