An Improved Clone Selection Method

  • Uros Jamnikar
  • Marjanca Blas
  • Dominik Gaser
  • Jerica Rozman-Pungercar
  • Andrej Francky
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 5)

Abstract

For commercial production of recombinant proteins and antibodies it is very important to obtain high producing cell clones. Due to this demand it is inevitable to introduce cell line development methods that enable screening of more clones and increase chance to isolate clones with best characteristics. It is hard and time consuming to find high producing clones using standard cloning methods as limited dilution. In most cases there are no resources to isolate and analyze more than few 100 clones. ClonePix FL (CP-FL, Genetix) technology substantially increases the number of screened clones in the selected pool before expansion in microtiter plates. In this study we wanted to compare productivity of clones obtained with limited dilution method and those obtained with ClonePix FL technology.

Keywords

Microtiter Plate Average Titer Parental Cell Line Light Imaging White Light Imaging 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Uros Jamnikar
    • 1
  • Marjanca Blas
    • 2
  • Dominik Gaser
    • 2
  • Jerica Rozman-Pungercar
    • 2
  • Andrej Francky
    • 2
  1. 1.Biopharmaceuticals-Cell and Molecular BiologyLek Pharmaceuticals d.d., Menges SiteMengesSlovenia
  2. 2.Biopharmaceuticals-Cell and Molecular BiologyLek Pharmaceuticals d.d., Menges SiteMengesSlovenia

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