The isolation and purification of plant viruses are necessary for the initial identification and characterization of viruses, for the production of antisera, and for a multitude of other studies. The terms “isolation” and “purification” often are used interchangeably by virologists to describe separation of virus particles in a pure form, free from plant host constituents. In this chapter the term “purification” will be used to describe this activity. The term “isolation” will be used to describe the pure culture isolation of a single virus from a mixture of co-infecting viruses.


Potato Virus Tomato Spotted Wilt Virus Virus Suspension Beet Necrotic Yellow Vein Virus Cesium Chloride 
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Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • P. E. Thomas
    • 1
  • W. K. Kaniewski
    • 2
  1. 1.Vegetable and Forage Crop Production, Agricultural Research Service, U.S. Department of AgricultureProsserUSA
  2. 2.Monsanto Co.St. LouisUSA

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