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Saccharomyces cerevisiae as a Model Organism for Elucidating Arsenic Tolerance Mechanisms

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Cellular Effects of Heavy Metals

Abstract

The budding yeast Saccharomyces cerevisiae is a powerful eukaryotic model organism for elucidating arsenic detoxification and tolerance acquisition mechanisms. The discovery of key yeast proteins involved in arsenite accumulation and efflux, arsenate reduction, and the use of complementation assays where a yeast protein is replaced by a homologous protein from another organism, has accelerated the identification of arsenic tolerance genes in fungi, plants, animals, and humans. In this chapter, we review the molecular biology of arsenic tolerance in budding yeast, focusing on arsenic sensing, signalling and detoxification mechanisms, how these pathways are regulated, and on the importance of yeast as a model for understanding fundamental aspects of arsenic tolerance in eukaryotes.

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Acknowledgements

The work in the Tamás lab is supported by the Swedish Research Council and the foundations Magnus Bergvalls Stiftelse and Stiftelsen Olle Engkvist Byggmästare. Work in the Wysocki lab is supported by grants from the Polish Ministry of Science and Higher Education.

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Wysocki, R., Tamás, M.J. (2011). Saccharomyces cerevisiae as a Model Organism for Elucidating Arsenic Tolerance Mechanisms. In: Banfalvi, G. (eds) Cellular Effects of Heavy Metals. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-0428-2_4

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