Fluorescence Spectroscopy and Energy Transfer Processes in Biological Systems
This article is divided into three parts. In the first part we review the fundamental principles of fluorescence spectroscopy, starting with the consideration of fluorophores and of the characteristics of fluorescence spectroscopy. The processes of fluorescence quenching, fluorescence anisotropy, and resonance energy transfer are presented, together with the information they can provide. The techniques that produce absorption spectra, excitation spectra, fluorescence under continuous excitation and response to pulsed excitation are also examined.
In the second part the basic interactions between atoms are introduced by considering first the static and then the dynamic effects of these interactions in a two-atom system ad in a linear chain of atoms. Subsequently the different types of interactions (multipolar electric and magnetic, and exchange) are examined. After a review of the different modes of excitation of a system containing both donors and acceptors, a statistical treatment of energy transfer is presented by considering first the case of energy transfer without migration among donors, and then the case when such migration occurs.
In the third part the concepts presented in the second part are applied to distance distribution analysis and FRET (Fluorescence Resonance Energy Transfer) in biological systems.
KeywordsEnergy Transfer Fluorescence Spectroscopy Fluorescence Resonance Energy Transfer Fluorescence Anisotropy Energy Transfer Process
Unable to display preview. Download preview PDF.
- 1.3Gregory J. (ed.) (1966) Handbook of fluorescent probes and research products, 9th ed., Molecular Probes Inc., Eugene, ORGoogle Scholar
- 1.7Berlman I. B. (1971), Handbook of aromatic molecules, 2nd ed., Academic, New YorkGoogle Scholar
- 1.8Stern O. and Volmer M. (1919), Phys. Z. 20, 183Google Scholar
- 1.11Berberan-Santos M. N. (2001), in New Trends in Fluorescence Spectroscopy: Applications to Chemical and Life Sciences, 18, 733, B. Valeur and J.C. Brochon eds, Springer, New YorkGoogle Scholar
- 2.1Eyring H., Walter J and Kimball G. F. (1944), Quantum Chemistry, Wiley, New York, p. 351Google Scholar
- 2.5Watts R. K. (1975), in Optical Properties of Ions in Solids, B. Di Bartolo ed., Plenum Press, New York and London, p. 307Google Scholar
- 2.6Perrin F. (1928), Compt. Rend. 178, 1978Google Scholar
- 2.7Stern O. and Volmer M. (1919), Physik Z. 20, 183Google Scholar
- 2.8Förster Th. (1949), Z. Naturforsch 4a, 321Google Scholar
- 2.10Galanin M. D, (1955), Sov. Phys. JETP 1, 317Google Scholar
- 2.11Reif F. (1965), Fundamentals of Statistical and Thermal Physics, McGraw Hill, New York, p.483Google Scholar
- 2.13Watts R. K. and Richter H. J. (1972), Phys. Rev. B6, 1584Google Scholar
- 3.2Clegg R. M. (1966), in Fluorescence Imaging Spectroscopy, X. F. Wang and B. Herman, eds., p. 179, Wiley, New YorkGoogle Scholar
- 3.3Yokota M. and Tanimoto O. (1967), J. Phys. Soc. Japan 22(3), 779Google Scholar