Nanoscopy Using Localization and Temporal Separation of Fluorescence From Single Molecules

  • C. Steinhauer
  • C. Forthmann
  • R. Jungmann
  • J. Vogelsang
  • F. C. Simmel
  • P. Tinnefeld
Conference paper
Part of the NATO Science for Peace and Security Series B: Physics and Biophysics book series (NAPSB)


Since the development of the first techniques [1, 2] that bypass the diffraction limit postulated by Ernst Abbe in 1873, so called superresolution microscopies have spread rapidly diverging into different variants that are capable of resolving details smaller than 200 nm. This article focuses on those based on the subsequent localization of single molecules. Therein, most molecules are prepared in a non-fluorescent dark state, leaving only a few single molecules fluorescing, which can be localized by fitting a two dimensional Gaussian function to their respective point spread functions (PSF).


Single Molecule Temporal Separation Localization Precision Point Spread Function Superresolution Image 
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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • C. Steinhauer
    • 1
  • C. Forthmann
    • 1
  • R. Jungmann
    • 2
  • J. Vogelsang
    • 1
  • F. C. Simmel
    • 2
  • P. Tinnefeld
    • 1
  1. 1.Angewandte Physik – Biophysik & Center for NanoscienceLudwig-Maximilians-Universität MünchenMünchenGermany
  2. 2.Physics Department E14 & Center for NanoscienceTechnische Universität MünchenGarchingGermany

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