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Principles and Applications of Fluorescence Correlation Spectroscopy (FCS)

  • Petra Schwille
  • Jonas Ries
Conference paper
Part of the NATO Science for Peace and Security Series B: Physics and Biophysics book series (NAPSB)

Abstract

Fluorescence correlation spectroscopy (FCS) is one of the various modern optical techniques that allow access to single fluorescently labeled biomolecules in aqueous solution. In contrast to other fluorescence measurements, however, the parameter of primary interest is not the emission intensity itself, but rather spontaneous intensity fluctuations caused by the minute deviations of a small ensemble of molecules from thermal equilibrium. In general, all physical parameters that give rise to fluctuations in the fluorescence signal are accessible by FCS. It is, for example, rather straightforward to determine local concentrations, mobility coefficients or characteristic rate constants of inter- or intramolecular reactions of fluorescently labeled biomolecules at nanomolar to micromolar concentrations.

Keywords

Fluorescence Correlation Spectroscopy Detection Volume Correlation Curve Focal Volume Autocorrelation Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Biophysics GroupBIOTEC TU DresdenDresdenGermany

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