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Functional Assays for Ricin Detection

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Detection of Biological Agents for the Prevention of Bioterrorism

Abstract

In this review, we provide background information on ricin structure, present available functional assays for other toxins that are potential biothreat agents, and finish by describing the functional assay of ricin itself. Using appropriate sample preparation and optimized detection based on N-glycosidase activity, we demonstrate that specific detection of whole ricin at a level of around 0.1 ng/mL is possible and applicable to environmental samples.

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Authors and Affiliations

  1. CEA, Ibitecs, Service de Pharmacologie et d’Immunoanalyse, 911191, Gif-sur-Yvette, France

    Eric Ezan, Elodie Duriez, François Fenaille & François Becher

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  1. Eric Ezan
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  2. Elodie Duriez
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  3. François Fenaille
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  4. François Becher
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Correspondence to Eric Ezan .

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Editors and Affiliations

  1. , Science Branch, Fisheries and Oceans Canada, St. John's, A1C 5X1, Newfoundland and Labrador, Canada

    Joseph Banoub

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Ezan, E., Duriez, E., Fenaille, F., Becher, F. (2011). Functional Assays for Ricin Detection. In: Banoub, J. (eds) Detection of Biological Agents for the Prevention of Bioterrorism. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9815-3_9

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