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Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation

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Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

Part of the book series: Developments in Applied Phycology ((DAPH,volume 4))

Abstract

This chapter investigates the use of chlorophyll a fluorescence to better understand changes in the regulation of photosynthetic electron transport. It describes the different electron pathways utilised by photosynthetic organisms, including pathways used in photosynthesis as well as alternative electron cycling (AEC). The major photoprotective processes are described, in particular, non-photochemical quenching (NPQ) and its three components, energy-dependent quenching (qE), state-transition quenching (qT), and photoinhibition (qI). Fluorescence and NPQ responses to light stress are compared across a higher plant, diatom and cyanobacteria. Photosynthesis is a complex interaction of complementary processes making the identification and isolation of a particular photosynthetic pathway or process inherently difficult. Therefore, we describe the use of chemicals which allow for the differentiation of mechanistic photosynthetic processes, such as electron transport pathways, CO2 fixation and the use of trans-thylakoid proton gradients, which can be effectively understood and quantified using chlorophyll fluorescence detection techniques.

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Acknowledgement

PR thanks the Australian Research Council for financial support. CW and TJ thank the Deutsche Forschungsgemeinschaft for financial support Wi 764/12. JL thanks the Centre National de la Recherche Scientifique (CNRS), the Agence Nationale de la Recherche (ANR program VasiRéMi) and the Deutsche Forschungsgemeinschaft (DFG, grant LA2368/2-1) for their financial support. Ross Hill is thanked for editorial comments.

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Authors and Affiliations

  1. Plant Function Biology & Climate Change Cluster, University of Technology, 123, Broadway, Sydney, NSW, Austraila

    Peter J. Ralph & Katherina Petrou

  2. Department of Plant Physiology, University of Leipzig, D-04103, Leipzig, Johannisallee 23, Germany

    Christian Wilhelm & Torsten Jakob

  3. CNRS UMR6250 ‘LIENSs’, Institute of Coastal and Environmental Research (ILE), University of La Rochelle, La Rochelle, France

    Johann Lavaud

  4. Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570, Bremerhaven, Germany

    Sven A. Kranz

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  1. Peter J. Ralph
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  2. Christian Wilhelm
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Correspondence to Peter J. Ralph .

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  1. , Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, United Kingdom

    David J. Suggett

  2. Institute of Microbiology, Opatovciky mlyn, Trebon, 379 81, Czech Republic

    Ondrej Prášil

  3. Murdoch University, School of Biological Sciences and Biotec, Algae R&D Center, Murdoch, 6150, West Australia, Australia

    Michael A. Borowitzka

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Ralph, P.J., Wilhelm, C., Lavaud, J., Jakob, T., Petrou, K., Kranz, S.A. (2010). Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation. In: Suggett, D., Prášil, O., Borowitzka, M. (eds) Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Developments in Applied Phycology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9268-7_4

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