Overview of Fluorescence Protocols: Theory, Basic Concepts, and Practice

  • Yannick Huot
  • Marcel Babin
Part of the Developments in Applied Phycology book series (DAPH, volume 4)


At the request of the editors, this chapter is based on the book section written by Babin (2008), however, much of the manuscript has been revised and updated to address different readerships. While Babin (2008) is aimed at a more general audience interested in understanding the basis of the measurement and the current instruments available, this chapter is aimed at those who will use the fluorescence tool and are interested in understanding more of its underlying theory, as well as the assumptions associated with it. In short, while the first chapter was aimed more at a beginning user, this one is aimed more at an intermediate user. Nevertheless, some sections have seen little changes.


Quantum Yield Reaction Centre Photosynthetic Pigment Charge Separation Colored Dissolve Organic Matter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are very thankful to John Cullen for sharing with us his insights on chlorophyll fluorescence over the years. This work was funded by a CNES grant to M. Babin and a CNES fellowship to Y. Huot.


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© Springer Netherlands 2010

Authors and Affiliations

  1. 1.Département de Géomatique AppliquéeUniversité de SherbrookeSherbrookeCanada
  2. 2.Laboratoire d’Océanographie de Villefranche, UMR 7093Université Pierre et Marie Curie-Paris 6ParisFrance

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