Abstract
Chlorophyll (Chl) fluorescence has become one of the most common and useful techniques in photosynthetic field research. Its non-invasiveness, sensitivity and the wide availability of reliable instruments, also makes it a convenient and suitable technique in microalgal biotechnology to monitor a culture’s photosynthetic performance. Experimentally, homogenous microalgal cultures in suspension have also been ideal objects in photosynthetic studies. For the purpose of this book we summarised results of experiments since the 1990s that have pioneered the practical use of Chl fluorescence methods to monitor the physiological status of fast-growing microalgal mass cultures, optimising and estimating biomass productivity or finding marker processes of certain compound synthesis.
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References
Baker NR (2008) Chlorophyll Fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113
Behrenfeld MJ, Prasil O, Kolber ZS, Babin M, Falkowski PG (1998) Compensatory changes in photosystem II electron turnover rates protect photosynthesis from photoinhibition. Photosynth Res 58:259–268
Ben-Amotz A (2004) Industrial production of microalgal cell-mass and secondary products – Major industrial species: Dunaliella. In: Richmond A (ed) Handbook of microalgal mass cultures. Blackwell Science, Oxford, pp 273–280
Benemann JR, Oswald WJ (1996) Systems and economic analysis of microalgae ponds for conversion of CO2 to biomass. Final report. US DOE http://www.osti.gov/bridge/servlets/purl/493389-FXQyZ2/webviewable/493389.pdf
Bilger W, Björkman O (1990) Role of xanthophyll in photoprotection elucidated by measurement of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynth Res 25:173–185
Boardman NK (1980) Energy from the biological conversion of solar energy. Phil Trans R Soc London A 295:477–489
Borowitzka MA (2005) Carotenoid production using microorganisms. In: Cohen Z, Ratledge C (eds) Single Cell Oils. AOCS Press, Champaign, IL, pp 124–137
Bradbury M, Baker NR (1984) A quantitative determination of photochemical and nonphotochemical quenching during the slow phase of the chlorophyll fluorescence induction curve of bean leaves. Biochim Biophys Acta 765:275–81
Büchel C, Wilhelm C (1993) In vivo analysis of slow chlorophyll fluorescence induction kinetics in algae: progress problems and perspectives. Photochem Photobiol 58:137–148
Casper-Lindley C, Björkman O (1998) Fluorescence quenching in four unicellular algae with different light-harvesting and xanthophyll-cycle pigments. Photosynth Res 56:277–289
Demmig B, Winter K, Krüger A, Czygan FC (1987) Photoinhibition and zeaxanthin formation in intact leaves. Plant Physiol 84:218–224
Demmig-Adams B (1990) Carotenoids and photoprotection in plants. A role for the xanthophyll zeaxanthin. Biochim Biophys Acta 1020:1–24
Diner BA (1998) Photosynthesis: molecular biology of energy capture. Methods Enzymol 297:337–360
Finazzi G, Johnson GN, Dall’Osto L, Joliot P, Wollman F-A, Bassi R (2004) A zeaxanthin-independent nonphotochemical quenching mechanism localized in the photosystem II core complex. Proc Nat Acad Sci USA 101:12375–12380
Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92
Gilmore AM, Yamamoto HY (1991) Zeaxanthin formation and energy dependent fluorescence quenching in pea chloroplasts under artificially mediated linear and cyclic electron transport. Plant Physiol 96:635–643
Govindjee (1995) Sixty-three years since Kautsky: chlorophyll a fluorescence. Aust J Plant Physiol 22:131–160
Grobbelaar JU (2007) Photosynthetic characteristics of Spirulina platensis grown in commercial-scale open outdoor raceway ponds: what do the organisms tell us? J Appl Phycol 19:591–598
Hofstraat JW, Peeters JCH, Snel JFH, Geel C (1994) Simple determination of photosynthetic efficiency and photoinhibition of Dunaliella tertiolecta by saturating pulse fluorescence measurements. Mar Ecol Prog Ser 103:187–196
Jin ES, Yokthongwattana K, Polle JEW, Melis A (2003) Role of the reversible xanthophylls cycle in the Photosystem II damage and repair cycle in Dunaliella salina. Plant Physiol 132:352–364
Kitajima M, Butler WL (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta 376:105–115
Klughammer C, Schreiber U (2008) Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the saturation pulse method. PAM Application Notes 1: 27–35. http://www.walz.com/e_journal/pdfs/PAN078007.pdf
Krause GH (1988) Photoinhibition of photosynthesis. An evaluation of damaging and protecting mechanisms. Physiol Plant 74:566–574
Kirilovsky D (2007) Photoprotection in cyanobacteria: the orange carotenoid protein (OCP)-related non-photochemical-quenching mechanism. Photosynth Res 93:7–16
Lu CM, Vonshak A (1999) Photoinhibition in outdoor Spirulina platensis cultures assessed by polyphasic chlorophyll fluorescence transients. J Appl Phycol 11:355–359
Masojídek J, Torzillo G, Koblížek M, Kopecký J, Bernardini P, Sacchi A, Komenda J (1999) Photoadaptation of two members of the Chlorophyta (Scenedesmus and Chlorella) in laboratory and outdoor cultures: changes in chlorophyll fluorescence quenching and the xanthophyll cycle. Planta 209:126–135
Masojídek J, Torzillo G, Kopecký J, Koblížek M, Nidiaci L, Komenda J, Lukavská A, Sacchi A (2000) Changes in chlorophyll fluorescence quenching and pigment composition in the green alga Chlorococcum sp. grown under nitrogen deficiency and salinity stress. J Appl Phycol 12:417–426
Masojídek J, Grobbelaar JU, Pechar L, Koblížek M (2001) Photosystem II electron transport rate and oxygen production in natural waterblooms of freshwater cyanobacteria during the diel cycle. J Plankton Res 23:57–66
Masojídek J, Sergejevová M, Rottnerová K, Jirka V, Korečko J, Kopecký J, Zaťková I, Torzillo G, Štys D (2009) A two-stage solar photobioreactor for cultivation of microalgae based on solar concentrators. J Appl Phycol 21:55–63
Masojídek J, Koblížek M, Torzillo G (2004a) Photosynthesis in microalgae. In: Richmond A (ed) Handbook of microalgal mass cultures. Blackwell Science, Oxford, pp 20–39
Masojídek J, Kopecký J, Koblížek M, Torzillo G (2004b) The xanthophyll cycle in green algae (Chlorophyta): its role in the photosynthetic apparatus. Plant Biol 6:342–349
Masojídek J, Sergejevová M, Rottnerová K, Jirka V, Korečko J, Kopecký J, Zaťková I, Torzillo G, Štys D (2009) A two-stage solar photobioreactor for cultivation of microalgae based on solar concentrators. J Appl Phycol 21:55–63
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence – a practical guide. J Exp Bot 51:659–668
Neale J (1987) Algal photoinhibition and photosynthesis in the aquatic environment. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) Photoinhibition. Elsevier Science, Amsterdam, pp 39–65
Nedbal L, Tichy V, Xiong F, Grobbelaar JU (1996) Microscopic green algae and cyanobacteria in high-frequency intermittent light. J Appl Phycol 8:325–333
Neubauer C, Schreiber U (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination. I. Saturation characteristics and partial control by the Photosystem II acceptor side. Z Naturforsch 42c:1246–1254
Pirt SJ (1986) The thermodynamic efficiency (quantum demand) and dynamics of photosynthetic growth. New Phytol 102:3–37
Prášil O, Kolber Z, Berry JA, Falkowski PG (1996) Cyclic electron flow around photosystem II in vivo. Photosynth Res 48:395–410
Pulz O, Scheibenboden K, Gross W (2001) Biotechnology with cyanobacteria and microalgae. In: Reed G (ed) Special processes: biotechnology, vol 10. Wiley-VCH, Weinheim, pp 107–136
Ramus J (1981) The capture and transduction of light energy. In: Lobban CS, Wynne MJ (eds) The biology of seaweeds. Blackwell Scientific, Oxford, pp 458–92
Richmond A (2000) Microalgal biotechnology at the turn of the millenium: a personal view. J Appl Phycol 12:441–451
Richmond A (2004) Biological principles of mass cultivation. In: Richmond A (ed) Handbook of microalgal mass cultures. Blackwell Science, Oxford, pp 125–177
Schreiber U (2004) Pulse-Amplitude-Modulation (PAM) fluorometry and saturation pulse method: an overview. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Dordrecht, pp 279–319
Schreiber U, Neubauer C (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: II. Partial control by the photosystem II donor side and possible ways of interpretation. Z Naturforsch 42c:1255–1264
Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and nonphotochemical fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10:51–62
Schreiber U, Endo T, Mi H, Asada K (1995) Quenching analysis of chlorophyll fluorescence by the saturation pulse method: particular aspects relating to the study of eukaryotic algae and cyanobacteria. Plant Cell Physiol 36:873–882
Schreiber U, Bilger W, Hormann H, Neubauer C (1998) Chlorophyll fluorescence as a diagnostic tool: basics and some aspects of practical relevance. In: Raghavendra AS (ed) Photosynthesis: a comprehensive treatise. Cambridge University Press, Cambridge, pp 320–334
Srivastava AM, Strasser RJ, Govindjee (1999) Greening of pea leaves: parallel measurement of 77K emission spectra, OJIP chlorophyll a fluorescence transient, period four oscillation of the initial fluorescence level, delayed light emission, and P700. Photosynthetica 37:365–392
Strasser BJ, Strasser RJ (1995) Measuring fast fluorescence transients to address environmental questions: the JIP test. In: Mathis P (ed) Photosynthesis from light to biosphere, vol 5. Kluwer, Dordrecht, pp 977–980
Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol 61:33–42
Strasser RJ, Tsimili-Michael M, Srivastava A (2004) Analysis of the Chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer, Dordrecht, pp 321–362
Sukenik A, Bennet J, Falkowski PG (1987) Light-saturated photosynthesis – limitation by electron transport or carbon fixation? Biochim Biophys Acta 891:205–215
Sukenik A, Beardall J, Kromkamp JC, Kopecký J, Masojídek J, van Bergeijk S, Gabai S, Shaham E, Yamshon A (2009) Photosynthetic performance of outdoor Nannochloropsis mass cultures to extreme environmental conditions – assessment by chlorophyll fluorescence techniques. Aquat Microb Ecol 56: 297–308
Ting CS, Owens TG (1992) Limitation of the pulse-modulated technique for measuring the fluorescence characteristics of algae. Plant Physiol 100:367–373
Torzillo G, Pushparaj B, Masojidek J, Vonshak A (2003). Biological constraints in algal biotechnology. Biotechnol Bioprocess Engineering, 8:338–348
Torzillo G, Accolla P, Pinzani E, Masojídek J (1996) In situ monitoring of chlorophyll fluorescence to assess the synergistic effect of low temperature and high irradiance stresses in Spirulina cultures grown outdoors in photobioreactors. J Appl Phycol 8:283–291
Torzillo G, Bernardini P, Masojídek J (1998) On-line monitoring of chlorophyll fluorescence to assess the extent of photoinhibition of photosynthesis induced by high oxygen concentration and low temperature and its effect on the productivity of outdoor cultures of Spirulina platensis (Cyanobacteria). J Phycol 34:504–510
Torzillo G, Goksan T, Faraloni C, Kopecky J, Masojídek J (2003) Interplay between photochemical activities and pigment composition in an outdoor culture of Haematococcus pluvialis during the shift from the green to red stage. J Appl Phycol 15:127–136
Tredici M (2004) Mass production of microalgae: photobioreactors. In: Richmond A (ed) Handbook of microalgal mass cultures. Blackwell Science, Oxford, pp 178–214
Van Kooten O, Snel JFH (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res 25:147–145
Vonshak A, Torzillo G (2004) Environmental stress physiology. In: Richmond A (ed) Handbook of microalgal mass cultures. Blackwell Science, Oxford, pp 57–82
Vonshak A, Torzillo G, Tomaselli L (1994) Use of chlorophyll fluorescence to estimate the effect of photoinhibition in outdoor cultures of Spirulina platensis. J Appl Phycol 6:31–34
Vonshak A, Torzillo G, Accolla P, Tomaselli L (1996) Light and oxygen stress in Spirulina platensis (cyanobacteria) grown outdoors in tubular reactors. Physiol Plant 97:175–179
Vonshak A, Torzillo G, Masojídek J, Boussiba S (2001) Sub-optimal morning temperature induces photoinhibition in dense outdoor cultures of the alga Monodus subterraneus (Eustigmatophyta). Plant Cell Environ 24:1113–1118
Walker DA (2009) Biofuels, facts, fantasy, and feasibility. J Appl Phycol 21:509–517
Wollman F-A (2001) State transitions reveal the dynamics and flexibility of the photosynthetic apparatus. The EMBO Journal 20:3623–3630
Zhu X, Long SP, Ort DR (2008) Converting solar energy into crop production. Curr Opin Biotechnol 19:153–159
Acknowledgement
The authors thank Mr. Pavel Souček for preparation of diagrams and Mr. Steve Ridgill for language corrections.
The Ministry of Education, Youth and Sports and the Czech Academy of Sciences supported this work through the project MSM6007665808 and AVOZ 50200510. Partial funding was also provided by project 522/06/1090 and 521/09/0656 of the Czech Science Foundation, and by project IAA608170601 of the Grant Agency of the Czech Academy of Sciences.
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Masojídek, J., Vonshak, A., Torzillo, G. (2010). Chlorophyll Fluorescence Applications in Microalgal Mass Cultures. In: Suggett, D., Prášil, O., Borowitzka, M. (eds) Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Developments in Applied Phycology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9268-7_13
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