Which Microbial Communities Are Present? Using Fluorescence In Situ Hybridisation (FISH): Microscopic Techniques for Enumeration of Troublesome Microorganisms in Oil and Fuel Samples
Enumeration of microbes involved in souring of oil fields and microbiologically influenced corrosion (MIC) with culture-based methods, usually yield inadequate and contradictory results. Any cultivation step will almost certainly alter the population structure of the sample and thus the results of cultivation analysis are not a good basis for mitigation decisions. The need for methods that are cultivation independent has over the past 10 years facilitated the development of several analytical methods for determination of bacterial identity, quantity, and to some extent function, applied directly to samples of the native population. In this chapter, we demonstrate the features and benefits of applying microscopic techniques to a situation often encountered in the oil and petroleum industry: Control of microbial growth in fuel storage tanks. The methods described in this chapter will focus on direct counts of specific groups of microorganisms with microscopy and these are based on the detection of genetic material and not on culturing.
KeywordsTotal Cell Number Planktonic Cell Petroleum System Active Bacterium Microbiologically Influence Corrosion
Laboratory experiments and field monitoring were sponsored by DUC Partners (A.P. Møller-Mærsk, Shell and Chevron).
- Amann R, Ludwig W, Schleifer K-H (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Appl Environ Microbiol 59:143–169Google Scholar
- Beeder J, Nilsen RK, Rosnes JT, Torsvik T, Lien T (1994) Archaeoglobus fulgidus isolated from hot North Sea oil field waters. Appl Environ Microbiol 60:1227–1231Google Scholar
- Larsen J, Zwolle S, Kjellerup BV, Frølund B, Nielsen JP, Nielsen PH (2005) Identification of bacteria causing souring and biocorrosion in the Halfdan field by application of new molecular techniques Corrosion 2005, Paper 05629 (Houston, TX: NACE International, 2005).Google Scholar
- Larsen J, Skovhus TL, Agerbæk M, Thomsen TR, Nielsen PH (2006) Bacterial diversity study applying novel molecular methods on Halfdan produced waters Corrosion 2006, Paper 06668 (Houston, TX: NACE International, 2006).Google Scholar