Vascular Regeneration: Endothelial Progenitor Cell Therapy for Ischemic Diseases

  • Atsuhiko Kawamoto
  • Masaaki Ii
  • Takayuki Asahara


Since the discovery of circulating endothelial progenitor cells (EPCs) in adult human peripheral blood, EPCs are believed to home to sites of neovascularization, where they contribute to vascular regeneration by forming a structural component of capillaries and by secreting angiogenic factors, thereby enhancing vascular and blood flow recovery in ischemic tissue. This therapeutic strategy has been effective in animal models of ischemia, and we and other clinical trials have demonstrated that it was safe and feasible for treatment of critical ischemic limb and cardiovascular diseases. However, the decline of EPCs in the peripheral blood and evidence that several disease states reduced EPC number and/or function have prompted the development of several strategies to overcome these limitations, including the administration of genetically modified EPCs that overexpress angiogenic growth factors. To optimize therapeutic outcomes, investigators must keep refining methods of EPC purification, expansion, and administration, and to develop techniques that overcome the intrinsic decline and phenotypic deficiencies of EPCs. In this chapter, we have illustrated EPC biology and the therapeutic potential of EPCs for vascular regeneration demonstrating our data of clinical study.


Vascular Endothelial Growth Factor Endothelial Progenitor Cell Critical Limb Ischemia Corneal Neovascularization Hindlimb Ischemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Netherlands 2011

Authors and Affiliations

  • Atsuhiko Kawamoto
    • 1
  • Masaaki Ii
    • 1
    • 2
  • Takayuki Asahara
    • 3
  1. 1.Regenerative Medicine unitInstitute of Biomedical Research and Innovation/RIKEN Center for Developmental BiologyKobeJapan
  2. 2.Department of PharmacologyOsaka Medical CollegeOsakaJapan
  3. 3.Group of Vascular Regeneration ResearchInstitute for Biomedical Research and Innovation (IBRI)/RIKEN Center for Developmental BiologyKobe-shi HyogoJapan

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