Metallo β Lactamases Gene blaimp, blaspm and blavim Detection by Multiplex Real-Time TaqMan Assay on the Smartcycler

  • Ian Carter


Gram-negative bacteria have a range of resistance mechanisms that they can use to evade the actions of carbapenems and other beta-lactams. The common form of resistance is either through lack of drug penetration (i.e., outer membrane protein (OMP) mutations and efflux pumps), hyperproduction of an AmpC-type beta-lactamase, and/or carbapenem-hydrolyzing beta-lactamases. Based on molecular studies, two types of carbapenem-hydrolyzing enzymes have been described: serine enzymes possessing a serine moiety at the active site, and metallo-β-lactamases (MBLs), requiring divalent cations, usually zinc, as metal cofactors for enzyme activity. MBLs, like all β-lactamases, can be divided into those that are normally chromosomally mediated and those that are encoded by transferable genes. The early studies on chromosomally mediated MBLs mainly centered around Bacillus cereus (BC II), and Stenotrophomonas maltophilia (L1). However, primarily due to genomic sequencing, increasingly more chromosomally mediated genes are being discovered but are often found across a range of bacterial genera.


Bacillus Cereus Reaction Tube Serratia Marcescens Stenotrophomonas Maltophilia Enterobacter Aerogenes 
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  1. 1.
    Mendes R, Kiyota K, Monteiro J et al (2007) Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis. J Clin Microbiol 45(2):544–547PubMedCrossRefGoogle Scholar
  2. 2.
    Peleg A, Franklin C, Bell J et al (2004) Emergence of IMP-4 metallo-beta-lactamase in a clinical isolate from Australia. J Antimicrob Chemother 54:699–700PubMedCrossRefGoogle Scholar
  3. 3.
    Walsh T, Toleman M, Laurent Poirel et al (2005) Metallo-beta-Lactamases: the quiet before the storm? Clin Micro Rev 18(2):306–325PubMedCrossRefGoogle Scholar
  4. 4.
    Walsh T (2005) The emergence and implications of metallo-b-lactamases in Gram-negative bacteria. Clin Microbiol Infect 11 (Suppl 6): 2–9PubMedCrossRefGoogle Scholar
  5. 5. Section 5.5.8 (accessed 10 November 2009)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.SEALS Microbiology DivisionPrince of Wales HospitalRandwickAustralia

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