Advertisement

Entamoeba histolytica

  • Damien Stark
Chapter

Abstract

Entamoeba histolytica is morphologically identical to the non-pathogenic species Entamoeba dispar, and Entamoeba moshkovskii though genetic differences have confirmed the separation of these three as independent species [4, 13]. Due to this conserved morphology, stained smears of stool specimens are insufficient for differentiation of these species. While staining of fixed faecal smears can determine the presence of the Entamoeba histolytica/dispar/moshkovskii complex within a stool specimen, other techniques such as PCR or ELISA must be employed for differentiation. A number of PCR assays are available for detection and/or differentiation of Entamoeba species [3, 6, 11].

Keywords

TaqMan Assay Stool Specimen Entamoeba Histolytica Product Detection SYBR Green Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Becker GL Jr, Knep S, Lance KP et al (1980) Amebic abscess of the brain. Neurosurgery 6:192–194PubMedCrossRefGoogle Scholar
  2. 2.
    Calore EE, Calore NM, Cavaliere MJ (2002). Salpingitis due to Entamoeba histolytica. Braz J Infect Dis 6:97–99PubMedGoogle Scholar
  3. 3.
    Cruz-Reyes JA, Spice VM, Rehman T et al (1992) Ribosomal DNA sequences in the differentiation of pathogenic and non-pathogenic isolates of Entamoeba histolytica. Parasitology 104 (Pt 2):239–246PubMedCrossRefGoogle Scholar
  4. 4.
    Diamond LS, Clark CG (1993) A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. J Eukaryot Microbiol 40:340–344PubMedCrossRefGoogle Scholar
  5. 5.
    Evangelopoulos A, Legakis N, Vakalis N (2001) Microscopy, PCR and ELISA applied to the epidemiology of amoebiasis in Greece. Parasitol Int 50:185–189PubMedCrossRefGoogle Scholar
  6. 6.
    Hamzah Z, Petmitr S, Mungthin M et al (2006) Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assay. J Clin Microbiol 44:3196–3200PubMedCrossRefGoogle Scholar
  7. 7.
    Haque R, Mollah NU, Ali IK et al (2000) Diagnosis of amebic liver abscess and intestinal infection with the TechLab Entamoeba histolytica II antigen detection and antibody tests. J Clin Microbiol 38:3235–3239PubMedGoogle Scholar
  8. 8.
    Haque R, Neville LM, Hahn P et al (1995) Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. J Clin Microbiol 33:2558–2561PubMedGoogle Scholar
  9. 9.
    Knappik M, Borner U, Jelinek T (2005) Sensitivity and specificity of a new commercial enzyme-linked immunoassay kit for detecting Entamoeba histolytica IgG antibodies in serum samples. Eur J Clin Microbiol Infect Dis 24:701–703PubMedCrossRefGoogle Scholar
  10. 10.
    Qvarnstrom Y, James C, Xayavong M et al (2005) Comparison of real-time PCR protocols for differential laboratory diagnosis of amebiasis. J Clin Microbiol 43:5491–497PubMedCrossRefGoogle Scholar
  11. 11.
    Sanchez-Guillen Mdel C, Perez-Fuentes R, Salgado-Rosas H et al (2002) Differentiation of Entamoeba histolytica/Entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis. Am J Trop Med Hyg 66:731–737PubMedGoogle Scholar
  12. 12.
    Shamsuzzaman SM, Hashiguchi Y (2002) Thoracic amebiasis. Clin Chest Med 23:479–492PubMedCrossRefGoogle Scholar
  13. 13.
    Som I, Azam A, Bhattacharya A et al (2000) Inter- and intra-strain variation in the 5.8S ribosomal RNA and internal transcribed spacer sequences of Entamoeba histolytica and comparison with Entamoeba dispar, Entamoeba moshkovskii and Entamoeba invadens. Int J Parasitol 30:723–728PubMedCrossRefGoogle Scholar
  14. 14.
    Stauffer W, Abd-Alla M, Ravdin JI (2006) Prevalence and incidence of Entamoeba histolytica infection in South Africa and Egypt. Arch Med Res 37:266–269PubMedCrossRefGoogle Scholar
  15. 15.
    Stauffer W, Ravdin JI (2003) Entamoeba histolytica: an update. Curr Opin Infect Dis 16:479–485PubMedCrossRefGoogle Scholar
  16. 16.
    Troll H, Marti H, Weiss N (1997) Simple differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate-acetic acid-formalin concentration and PCR. J Clin Microbiol 35:1701–1705PubMedGoogle Scholar
  17. 17.
    van Hal SJ, Stark DJ, Fotedar R et al (2007) Amoebiasis: current status in Australia. Med J Aust 186:412–416PubMedGoogle Scholar
  18. 18.
    Verweij JJ, Vermeer J, Brien EA et al (2003) Entamoeba histolytica infections in captive primates. Parasitol Res 90:100–103PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Microbiology DepartmentSt Vincents HospitalDarlinghurstAustralia

Personalised recommendations