Laboratory diagnosis of cryptosporidiosis traditionally relies on special staining techniques, such as modified acid-fast, Kinyoun’s and Giemsa stains as oocysts are difficult to detect using basic light microsopy . Other alternative diagnostic techniques have also been employed. The use of ELISA has been described for the detection of Cryptosporidium antigen in stools . Various PCR assays, including conventional PCR, nested and RT-PCR have also been developed for the detection of Cryptosporidium species in stool specimens [2, 5, 6, 15]. Whilst being more expensive and time consuming, PCR has shown superior sensitivity for the detection of Cryptosporidium species compared to conventional staining, microscopy and ELISA [9, 12].
KeywordsHybridization Probe Giemsa Stain Cryptosporidium Species Conventional Staining Initial Amplification
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