• Damien Stark


Laboratory diagnosis of cryptosporidiosis traditionally relies on special staining techniques, such as modified acid-fast, Kinyoun’s and Giemsa stains as oocysts are difficult to detect using basic light microsopy [4]. Other alternative diagnostic techniques have also been employed. The use of ELISA has been described for the detection of Cryptosporidium antigen in stools [7]. Various PCR assays, including conventional PCR, nested and RT-PCR have also been developed for the detection of Cryptosporidium species in stool specimens [2, 5, 6, 15]. Whilst being more expensive and time consuming, PCR has shown superior sensitivity for the detection of Cryptosporidium species compared to conventional staining, microscopy and ELISA [9, 12].


Hybridization Probe Giemsa Stain Cryptosporidium Species Conventional Staining Initial Amplification 
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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Microbiology DepartmentSt Vincents HospitalDarlinghurstAustralia

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