Cryptosporidium

Chapter

Abstract

Laboratory diagnosis of cryptosporidiosis traditionally relies on special staining techniques, such as modified acid-fast, Kinyoun’s and Giemsa stains as oocysts are difficult to detect using basic light microsopy [4]. Other alternative diagnostic techniques have also been employed. The use of ELISA has been described for the detection of Cryptosporidium antigen in stools [7]. Various PCR assays, including conventional PCR, nested and RT-PCR have also been developed for the detection of Cryptosporidium species in stool specimens [2, 5, 6, 15]. Whilst being more expensive and time consuming, PCR has shown superior sensitivity for the detection of Cryptosporidium species compared to conventional staining, microscopy and ELISA [9, 12].

Keywords

Hybridization Probe Giemsa Stain Cryptosporidium Species Conventional Staining Initial Amplification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Microbiology DepartmentSt Vincents HospitalDarlinghurstAustralia

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