Tests such as ELISA that use antibodies against a mixture of norovirus strains are available commercially but lack specificity and sensitivity. RT-PCR assays are the most commonly used methods to detect noroviruses. PCR assays are targeted at conserved regions on either the polymerase or capsid genes. A variety of methods are described in the literature, however, no single assay is able to detect all known norovirus genogroups because of the high genetic variation. Here we describe two different RT-PCR assays [1–4].
KeywordsCapsid Gene Hunter Virus Primer G2F3 Human Genogroup