Human Polyomaviruses – JCV and BKV

  • Ian Carter
  • David M. Whiley


Detection of the human polyomaviruses JC and BK by the polymerase chain reaction (PCR) has been used to diagnose the neurological disease, progressive multifocal leukoencephalopathy, and to study viruria in immunocompromised and nonimmunosuppressed individuals. The universal nature of PCR technology now makes it possible for many laboratories to diagnose infections produced by these viruses.


Progressive Multifocal Leukoencephalopathy Progressive Multifocal Leukoencephalopathy Human Polyomavirus Haemorrhagic Cystitis Polymerase Chain Reaction Technology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Arthur RR, Dagostin S, Shah KV (1989) Detection of BKV and JCV in urine and brain tissue by the polymerase chain reaction. J Clin Microbiol 27:1174–1179PubMedGoogle Scholar
  2. 2.
    Arthur RR, Shah KV (1989) Occurrence and significance of papoviruses BK and JC in the urine. In: Melnick J (ed) Progress in Medical Virology, vol 36. Karger, Basel, pp 42–61Google Scholar
  3. 3.
    Bialasiewicz S, Whiley DM, Nissen MD et al (2007) The impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR. J Clin Microbiol 45:1621–1623PubMedCrossRefGoogle Scholar
  4. 4.
    Berger JR, Kaszovitz B, Donovan Post MJ, Dickinson G (1987) Clinical review: progressive multifocal leukoencephalopathy associated with human immunodeficiency virus infection. Arm Intern Med 107:78–87CrossRefGoogle Scholar
  5. 5.
    Chesters PM, Heritage J, McCance DJ (1983) Persistence of DNA sequences of BK virus and JC virus in normal human tissues and in diseased tissues. J Infect Dis 147:676–684PubMedCrossRefGoogle Scholar
  6. 6.
    Heritage J, Chesters PM, McCance DJ (1981) The persistence of papovavirus BK DNA sequences in normal human renal tissue. J Med Virol 8:143–150PubMedGoogle Scholar
  7. 7.
    Hirsch HH, Mohaupt M, Klimkait T (2001) Prospective monitoring of BK virus load after discontinuing sirolimus treatment in a renal transplant patient with BK virus nephropathy. J Infect Dis 184:1494–1495PubMedCrossRefGoogle Scholar
  8. 8.
    Pal A, Sirota L, Maudru T et al (2006) Real-time, quantitative PCR assays for the detection of virus-specific DNA in samples with mixed populations of polyomaviruses. J Virol Methods 135:32–42PubMedCrossRefGoogle Scholar
  9. 9.
    Walker D, Padgett BL (1983) Progressive multifocal leukoencephalopathy, In: Fraenkel Conrat H, Wagner RR (eds) Comprehensive Virology, vol 18. Plenum, New York, pp 161–193Google Scholar
  10. 10.
    Whiley DM, Arden KE, Mackay IM et al (2004) Simultaneous detection and differentiation of human polyomaviruses JC and BK by a Rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population. J Med Virol 72:467–472PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.SEALS Microbiology DivisionPrince of Wales HospitalRandwickAustralia
  2. 2.Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research CentreQueensland Children’s Medical Research Institute, Children’s Health Service DistrictBrisbaneAustralia

Personalised recommendations