Abstract
Haemophilus ducreyi is the causative agent of chancroid, and Klebsiella granulomatis is the cause of Donovanosis. Both are classified as agents of genital ulcer disease. This method describes a multiplex polymerase chain reaction targeting these organisms in which amplification products are detected by an enzyme linked amplicon hybridization assay.
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References
Mackay IM, Metharom P, Sloots TP et al (2001) Quantitative PCRELAHA for the determination of retroviral vector transduction efficiency. Mol Ther 3:801–808
Mackay IM, Gardam T, Arden KE et al (2003) Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA. J Clin Virol 28:291–302
Mackay IM, Harnett G, Jeoffreys N et al (2006) Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers. Clin Infect Dis 42(10):1431–1438
Orle KA, Gates CA, Martin DH et al (1996) Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers.J Clin Microbiol 34:49–54
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Mackay, I.M. (2010). Haemophilus ducreyi and Klebsiella granulomatis. In: Schuller, M., Sloots, T., James, G., Halliday, C., Carter, I. (eds) PCR for Clinical Microbiology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9039-3_16
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DOI: https://doi.org/10.1007/978-90-481-9039-3_16
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