Diarrheagenic Escherichia coli Pathotypes (DEP) Including Enterohaemorrhagic (EHEC)/Shiga-toxin E. coli (STEC)

  • James Chin
  • Sam Abraham
  • Ren Zhang
  • Rafat Al-Jassim


Many methods are available for the detection and isolation of diarrheagenic Escherichia coli pathotypes (DEPs). A versatile protocol is one that will enable detection from any source material ranging from fecal samples, swabs, and foods or food products that have been processed in a stomacher.


Restriction Fragment Pattern Expanded Host Range Labile Toxin Bacterium Pellet Antigen Gene Cluster 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Chapman TA, Wu XY, Barchia I et al (2006) Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine. Appl Environ Microbiol 72:4782–4795PubMedCrossRefGoogle Scholar
  2. 2.
    Coimbra RS, Grimont F, Grimont PAD (1999) Identification of Shigella serotypes by restriction of amplified O-antigen gene cluster. Res Microbiol 150:543–553PubMedCrossRefGoogle Scholar
  3. 3.
    Coimbra RS, Grimont F, Burguière P et al (2000) Identification of Escherichia coli O-serogroups by restriction of the amplified O-antigen gene cluster (rfb-RFLP). Res Microbiol 151:639–654PubMedCrossRefGoogle Scholar
  4. 4.
    Hornitzky MA, Mercieca K, Bettelheim KA et al (2005) Bovine Feces from Animals with Gastrointestinal Infections Are a Source of Serologically Diverse Atypical Enteropathogenic Escherichia coli and Shiga Toxin-Producing E. coli Strains That Commonly Possess Intimin. Appl Environ Microbiol 71:3405–3412Google Scholar
  5. 5.
    Lehmacher A, Meier AH, Aleksic S et al (1998) Detection of hemolysin variants of shiga toxin-producing Escherichia coli by PCR and culture on vancomycin-cefixime-cefsulodin blood agar. Appl Environ Microbiol 64:2449–2453PubMedGoogle Scholar
  6. 6.
    Lopez-Saucedo C et al (2003) Single multiplex a polymerase chain reaction to detect diverse loci associated with a diarrheagenic Escherichia coli. Emerg Infect Dis 9:127–131PubMedCrossRefGoogle Scholar
  7. 7.
    Riley LW (2004) Distinguishing pathovars from nonpathovars: Escherichia coli, in Riley LW Molecular Epidemiology of Infectious Diseases: Principles and Practices. ASM Press, Washington DC, pp 179–206Google Scholar
  8. 8.
    Wu X-Y, Chapman T, Trott DJ et al (2007) Comparative analysis of virulence genes, genetic diversity, and phylogeny of commensal and enterotoxigenic Escherichia coli isolates from weaned pigs. Appl Environ Microbiol 73:83–91PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • James Chin
    • 1
  • Sam Abraham
    • 2
  • Ren Zhang
    • 2
  • Rafat Al-Jassim
    • 3
  1. 1.School of Veterinary Science, University of QueenslandBrisbaneAustralia
  2. 2.School of Biological Sciences, University of WollongongGwynnevilleAustralia
  3. 3.School of Animal Studies, University of QueenslandBrisbaneAustralia

Personalised recommendations