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Biological Application of Widefield Surface Plasmon Resonance Microscope to Study Cell/Surface Interactions and the Effect of TGF-β3, HCL and BSA/HCL on Cell Detachment Assay of Bone Cells Monolayer

  • Farshid SefatEmail author
  • Mansour Youseffi
  • Morgan Denyer
Chapter
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 60)

Abstract

Widefield Surface Plasmon Resonance (WSPR) microscope was used to investigate cell surface interactions under two different culture conditions: bone cells cultured on SPR substrate with transforming growth factor β3 (TGF-β3) and without as control. Trypsinisation was carried out in order to investigate its effect on cell detachment, in the presence of TGF-β3, HCl or BSA/HCl solutions. Trypsin was therefore added to four groups of bone cells with addition of TGF-β3, HCl, HCl/BSA solutions and one additional flask as control. These results further confirmed that application of TGF-β3, HCl and HCl/BSA decreased the degree of cell attachment on surface of culture flasks. HCl and BSA/HCl were tested as they are the carriers and solvents for TGF-β3. Cell detachment in control was about 43% after 6 min, which is slow. Bone cells in the presence of BSA/HCl started detaching from the surface about 4–5 min and cell detachment was about 63% after 6 min which was faster as compared to the control. Bone cells in the presence of HCl alone started detaching from the surface about 2 min (after applying trypsin) and cell detachment was about 69% after 6 min which was faster compared to the BSA/HCl and control. Trypsinisation experiments for bone cells cultured with TGF-β3 (50 ng/ml) showed that cells started to detach from the surface about 1 min after application of trypsin and cell detachment was about 85% after 4 min which was faster as compared to the control, HCl and BSA/HCl.

Keywords

WSPR imaging MG63 bone cell line BSA/HCl HCl TGF-β3 trypsinisation 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Farshid Sefat
    • 1
    Email author
  • Mansour Youseffi
    • 2
  • Morgan Denyer
    • 3
  1. 1.Institute of Pharmaceutical Innovation (ipi)University of BradfordBradfordUK
  2. 2.School of Engineering, Design and Technology-Medical EngineeringUniversity of BradfordBradfordUK
  3. 3.School of Life SciencesUniversity of BradfordBradfordUK

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