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Genotypic Differences in Symbiotic N2 Fixation of Some Alfalfa (Medicago sativa L.) Genotypes

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Sustainable use of Genetic Diversity in Forage and Turf Breeding

Abstract

In a highly controlled laboratory experiment, four alfalfa (Medicago sativa L.) cultivars were grown in all combinations with five strains of Sinorhizobium spp. with the aim to assess differences in nitrogen fixation among alfalfa genotypes and compatibility of symbionts. Based on shoot dry weight and total N content, it was noted that each cultivar showed a great compatibility with particular strains. Variety K-28 inoculated with all investigated strains exhibited the highest shoot dry matter, compared to other investigated cultivars. Using symbiotic N fixation, cultivar K-28 inoculated with L3Si strain gained high compatible association due to the 87% of shoot dry weight in relation to uninoculated control with full N content (100%). The significant coefficient of variation (CV%) for shoot dry matter of inoculated treatments was found (19.41–57.83%) depending on bacterial strain and plant genotype. Higher CV% values of inoculated plants compared to uninoculated plants indicated the influence of strain on increasing variability of the plants. As the effectiveness of the N2 fixation varies widely in different rhizobia-host combinations, it would be possible to identify the highly effective rhizobial strains, which would represent commercial strains of microbiological fertilizers for particular cultivars.

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References

  • Appunu, C., Sen, D., Singh, M.K., Dhar, B. 2008. Variation in symbiotic performance of Bradyrhizobium japonicum strains and soybean cultivars under field conditions. J. Cent. Eur. Agr. 9:185–189.

    Google Scholar 

  • Caetano-Anollés, G., Gresshoff, P. 1991. Plant genetic control of nodulation. Annu. Rev. Microbiol. 4:345–382.

    Article  Google Scholar 

  • Delić, D., Tomić, Z., Miličić, B., Radović, J., Stajković, O., Stanojković, A., Knežević-Vukčević, J. 2006. Evaluation of Sinorhizobium meliloti effect on N2 fixation variability in alfalfa. pp. 369–372. Proceedings of XXVI EUCARPIA Fodder Crops and Amenity Grasses Section and XVI Medicago spp. Perugia, Italy.

    Google Scholar 

  • Hefny, M., Dolinski, R., Malek, W. 2001. Variation in symbiotic characters of alfalfa cultivars inoculated with Sinorhizobium meliloti strains. Biol. Fertil. Soil 33:435–437.

    Article  Google Scholar 

  • Jessen, D.L., Barnes, D.K., Vance, C.P. 1988. Bidirectional selection in alfalfa for activity of nodule nitrogen and carbon-assimilating enzymes. Crop Sci. 28:18–22.

    Article  CAS  Google Scholar 

  • Mytton, L.R., Broockwell, J., Gibson, A.H. 1984. The potential for breeding an improved lucerne – Rhizobium symbiosis, 1st assessment of genetic variation. Euphytica 33:401–410.

    Article  Google Scholar 

  • Mytton, L.R., Skot, L. 1993. Breeding for improved symbiotic nitrogen fixation. In: Hayward, M.D., Bosemark, N.O., Romagosa, I. (eds.), Plant Breeding: Principles and Prospects (pp. 451–472). Chapman and Hall, London.

    Chapter  Google Scholar 

  • Phillips, A.D., Teuber, R.L. 1985. Genetic improvement of symbiotic nitrogen fixation in legumes. In: Evans, H.J., Bottomley, P.J., Newton, E.W. (eds.), Nitrogen Fixation Research Progress (pp. 11–17). Martinus Nijhoff, Dordrecht.

    Chapter  Google Scholar 

  • Rengel, Z. 2002. Breeding for better symbiosis. Plant Soil 245:147–162.

    Article  CAS  Google Scholar 

  • Vance, C.P. 1998. Legume symbiotic nitrogen fixation: agronomic aspects. In: Spaink, H.P., Kondorosi, A., Hooykaas, P.J.J. (eds.), The Rhizobiaceae (pp. 509–530). Kluwer, Dordrecht.

    Chapter  Google Scholar 

  • Vincent, M.J. 1970. A manual for the Practical Study of Root-Nodule Bacteria. In: IBP Handbook (Vol. 15). Blackwell, Oxford.

    Google Scholar 

  • Vitale, M., Pupilli, F., Scotti, C., Arcioni, S. 1996. Extent of RFLP and RAPD Variability in Tetraploid Populations of Alfalfa (pp. 305–308). Proceedings XVI EGF Meeting. Grado. Italy.

    Google Scholar 

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Acknowledgements

This research was supported by the Ministry of Science of the Republic of Serbia.

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Correspondence to Dušica Delić .

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Delić, D. et al. (2010). Genotypic Differences in Symbiotic N2 Fixation of Some Alfalfa (Medicago sativa L.) Genotypes. In: Huyghe, C. (eds) Sustainable use of Genetic Diversity in Forage and Turf Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8706-5_9

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