Dengue Virus Diagnostics

  • Evgeni Eltzov
  • Danit Atias
  • Levi Gheber
  • Robert S. Marks
Conference paper
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)


Dengue fever (DF) is an emerging arborviral disease caused by infection with dengue virus (DENV) which has emerged as the most important vector-borne viral disease in tropical areas and it continues to expand geographically. The four serotypes of DENV that cause human disease are transmitted by Aedes mosquitoes. Expansion in geographic distribution of both viruses and mosquito vectors, has led to a current global dengue pandemic. Dengue disease has a wide spectrum of expression: from asymptomatic infection or an influenza-like syndrome to severe disease including dengue hemorrhagic fever (DHF)/ dengue shock syndrome (DSS). Dengue infection diagnosis has come a long way and today, new techniques for detecting the virus and anti- DENV antibody are available. In spite of that there are still problems with those methods and a reliable diagnosis remains a crucial step towards the control of DENV disease in human populations. In this review, different diagnostic methods are described, including routinely used methods, newly developed techniques and commercially available kits. One of the limitations of current diagnostic tools for DENV is the lack of specificity and safety of the ­antigen employed as part of the available diagnostic tools. Thus there is a constant serological effort to develop alternative antigens that will help to improve the detection of anti-dengue antibodies and allow working with non hazardous materials. Several options to deal with this problem is discussed here.


Reverse Transcription Polymerase Chain Reaction West Nile Virus Dengue Virus Japanese Encephalitis Virus Dengue Fever 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Abd-Jamil J, Cheah CY, AbuBakar S (2008) Dengue virus type 2 envelope protein displayed as recombinant phage attachment protein reveals potential cell binding sites. Protein Engi Design Select 21(10):605–611CrossRefGoogle Scholar
  2. AnandaRao R, Swaminathan S, Fernando S, Jana AM, Khanna N (2005) A custom-designed recombinant multiepitope protein as a dengue diagnostic reagent. Protein Expr Purif 41(1):136–147PubMedCrossRefGoogle Scholar
  3. Arap MA (2005) Phage display technology - Applications and innovations. Gene Mole Biol 28:1–9CrossRefGoogle Scholar
  4. Atias D, Liebes Y, Chalifa-Caspi V, Bremand L, Lobel L, Marks SR, Dussart P (2009) Chemiluminescent optical fiber immunosensor for the detection of IgM antibody to Dengue virus in humans. Sensor Actuat B: Chemical 140:206–215CrossRefGoogle Scholar
  5. Baeumner AJ, Nicole AS, Naomi SS, Joseph R, Eun ML, Richard AM (2002) Biosensor for dengue virus detection: Sensitive, rapid, and serotype specific. Anal Chem 74:142–1448CrossRefGoogle Scholar
  6. Baeumner AJ, Schlesinger NA, Slutzki NS, Romano J, Lee EM, Montagna RA (2002b) Biosensor for Dengue virus detection: Sensitive, rapid, and serotype specific. Anal Chem 74(6):1442–1448PubMedCrossRefGoogle Scholar
  7. Balmaseda A, Guzman MG, Hammond S, Robleto G, Flores C, Tellez Y, Videa E, Saborio S, Perez L, Sandoval E, Rodriguez Y, Harris E (2003) Diagnosis of dengue virus infection by detection of specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva. Clin Diagn Lab Immunol 10(2):317–322PubMedGoogle Scholar
  8. Benhar I (2001) Biotechnological applications of phage and cell display. Biotechnol Adv 19(1):1–33PubMedCrossRefGoogle Scholar
  9. Cabezas S, Rojas G, Pavon A, Alvarez M, Pupo M, Guillen G, Guzman MG (2008) Selection of phage-displayed human antibody fragments on Dengue virus particles captured by a monoclonal antibody: application to the four serotypes. J Virol Methods 147(2):235–243PubMedCrossRefGoogle Scholar
  10. Callahan JD, Wu SJ, Dion-Schultz A, Mangold BE, Peruski LF, Watts DM, Porter KR, Murphy GR, Suharyono W, King CC, Hayes CG, Temenak JJ (2001) Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus. J Clin Microbiol 39(11):4119–4124PubMedCrossRefGoogle Scholar
  11. Cardosa MJ, Baharudin F, Hamid S, Hooi TP, Nimmanitya S (1995) A nitrocellulose membrane based IgM capture enzyme immunoassay for etiological diagnosis of dengue virus infections. Clin Diagn Virol 3(4):343–350PubMedCrossRefGoogle Scholar
  12. Cesareni G, Castagnoli L, Cestra G (1999) Phage displayed peptide libraries. Comb Chem High Throughput Screen 2(1):1–17PubMedGoogle Scholar
  13. Chambers TJ, Hahn CS, Galler R, Rice CM (1990) Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44:649–88PubMedCrossRefGoogle Scholar
  14. Chen RF, Yeh WT, Yang MY, Yang KD (2001) A model of the real-time correlation of viral titers with immune reactions in antibody-dependent enhancement of dengue-2 infections. FEMS Immunol Med Microbiol 30(1):1–7PubMedCrossRefGoogle Scholar
  15. Chow L, Hsu ST (1989) MAC-ELISA for the detection of IgM antibodies to dengue type I virus (rapid diagnosis of dengue type I virus infection). Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 22(4):278–285PubMedGoogle Scholar
  16. De Paula SO, Fonseca BA (2004) Dengue: a review of the laboratory tests a clinician must know to achieve a correct diagnosis. Braz J Infect Dis 8(6):390–398PubMedGoogle Scholar
  17. Diagnostics evaluation series No 3 (2009) Evaluation of commercially available anti dengue virus immunoglobulin M tests. Special programme for research and training in tropical diseases sponsored by WHO, p 1–42. ISBN 978 92 4 159775 3Google Scholar
  18. Dodeigne LCT, Lejeune R (2000) Chemiluminescence as diagnostic tool. A review. Talanta 51(3):415–439CrossRefGoogle Scholar
  19. Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, Gunther S (2002) Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol 40(7):2323–2330PubMedCrossRefGoogle Scholar
  20. Dussart P, Labeau B, Lagathu G, Louis P, Nunes MR, Rodrigues SG, Storck-Herrmann C, Cesaire R, Morvan J, Flamand M, Baril L (2006) Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol 13(11):1185–1189PubMedCrossRefGoogle Scholar
  21. Falconar AK, Young PR, Miles MA (1994) Precise location of sequential dengue virus subcomplex and complex B cell epitopes on the nonstructural-1 glycoprotein. Arch Virol 137(3–4):315–326PubMedCrossRefGoogle Scholar
  22. Garcia G, Vaughn DW, Del Angel RM (1997) Recognition of synthetic oligopeptides from nonstructural proteins NS1 and NS3 of dengue-4 virus by sera from dengue virus-infected children. Am J Trop Med Hyg 56(4):466–470PubMedGoogle Scholar
  23. Gentry MK, Henchal EA, McCown JM, Brandt WE, Dalrymple JM (1982) Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies. Am J Trop Med Hyg 31(3 Pt 1):548–555PubMedGoogle Scholar
  24. Gollins SW, Porterfield JS (1984) Flavivirus infection enhancement in macrophages: radioactive and biological studies on the effect of antibody on viral fate. J Gen Virol 65(Pt 8):1261–1272PubMedCrossRefGoogle Scholar
  25. Green S, Vaughn DW, Kalayanarooj S, Nimmannitya S, Suntayakorn S, Nisalak A, Lew R, Innis BL, Kurane I, Rothman AL, Ennis FA (1999) Early immune activation in acute dengue illness is related to development of plasma leakage and disease severity. J Infect Dis 179(4):755–762PubMedCrossRefGoogle Scholar
  26. Groen J, Koraka P, Velzing J, Copra C, Osterhaus AD (2000) Evaluation of six immunoassays for detection of dengue virus-specific immunoglobulin M and G antibodies. Clin Diagn Lab Immunol 7(6):867–871PubMedGoogle Scholar
  27. Gubler DJ (1998) Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11(3):480–496PubMedGoogle Scholar
  28. Gubler DJ (2002) Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol 10(2):100–3PubMedCrossRefGoogle Scholar
  29. Guzman MG, Kouri G (1996) Advances in dengue diagnosis. Clin Diagn Lab Immunol 3(6):621–627PubMedGoogle Scholar
  30. Guzman MG, Kouri G (2002) Dengue: an update. Lancet Infect Dis 2(1):33–42PubMedCrossRefGoogle Scholar
  31. Guzman MG, Kouri G, Martinez E, Bravo J, Riveron R, Soler M, Vazquez S, Morier L (1987) Clinical and serologic study of Cuban children with dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). Bull Pan Am Health Organ 21(3):270–279PubMedGoogle Scholar
  32. Harris E, Roberts TG, Smith L, Selle J, Kramer LD, Valle S, Sandoval E, Balmaseda A (1998) Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR. J Clin Microbiol 36(9):2634–2639PubMedGoogle Scholar
  33. Herrmann S, Leshem B, Landes S, Rager-Zisman B, Marks RS (2005) Chemiluminescent optical fiber immunosensor for the detection of anti-West Nile virus IgG. Talanta 66(1):6–14PubMedCrossRefGoogle Scholar
  34. Holmes EC, Twiddy SS (2003) The origin, emergence and evolutionary genetics of dengue virus. Infect Genet Evol 3(1):19–28PubMedCrossRefGoogle Scholar
  35. Hommel D, Talarmin A, Deubel V, Reynes JM, Drouet MT, Sarthou JL, Hulin A (1998) Dengue encephalitis in French Guiana. Res Virol 149(4):235–238PubMedCrossRefGoogle Scholar
  36. Houng HS, Chung-Ming Chen R, Vaughn DW, Kanesa-thasan N (2001) Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype-specific 3′ noncoding sequences. J Virol Methods 95(1–2):19–32PubMedCrossRefGoogle Scholar
  37. Huang MCY, Carlos FRM, Jonathan EF, Robert B, Brian TC, Connie JC-H (2008) VCSEL Optoelectronic biosensor for detection of infectious diseases. IEEE Photon Technol Lett 20(6)Google Scholar
  38. Huang MCY, Mateus CFR, Foley JE, Beatty R, Cunningham BT, Chang-Hasnain CJ (2008b) VCSEL optoelectronic biosensor for detection of infectious diseases. IEEE Photon Technol Lett 20(5–8):443–445CrossRefGoogle Scholar
  39. Hurrelbrink RJ, McMinn PC (2003) Molecular determinants of virulence: the structural and functional basis for flavivirus attenuation. Adv Virus Res 60:1–42PubMedCrossRefGoogle Scholar
  40. Innis BL, Nisalak A, Nimmannitya S, Kusalerdchariya S, Chongswasdi V, Suntayakorn S, Puttisri P, Hoke CH (1989) An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg 40(4):418–427PubMedGoogle Scholar
  41. Kao CL, King CC, Chao DY, Wu HL, Chang GJ (2005) Laboratory diagnosis of dengue virus infection: current and future perspectives in clinical diagnosis and public health. J Microbiol Immunol Infect 38(1):5–16PubMedGoogle Scholar
  42. Konry T, Novoa A, Shemer-Avni Y, Hanuka N, Cosnier S, Lepellec A, Marks RS (2005) Optical fiber immunosensor based on a poly(pyrrole-benzophenone) film for the detection of antibodies to viral antigen. Anal Chem 77(6):1771–1779PubMedCrossRefGoogle Scholar
  43. Kuberski TT, Rosen L (1977) A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. Am J Trop Med Hyg 26(3):533–537PubMedGoogle Scholar
  44. Kuhn RJ, Zhang W, Rossmann MG, Pletnev SV, Corver J, Lenches E, Jones CT, Mukhopadhyay S, Chipman PR, Strauss EG, Baker TS, Strauss JH (2002) Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 108(5):717–725PubMedCrossRefGoogle Scholar
  45. Kuno G (1998) Universal diagnostic RT-PCR protocol for arboviruses. J Virol Methods 72(1):27–41PubMedCrossRefGoogle Scholar
  46. Lam SK (1993) Rapid dengue diagnosis and interpretation. Malays J Pathol 15(1):9–12PubMedGoogle Scholar
  47. Lam SK, Devine PL (1998) Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produced during dengue infection. Clin Diagn Virol 10(1):75–81PubMedCrossRefGoogle Scholar
  48. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV (1992) Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30(3):545–551PubMedGoogle Scholar
  49. Laue T, Emmerich P, Schmitz H (1999) Detection of dengue virus RNA in patients after primary or secondary dengue infection by using the TaqMan automated amplification system. J Clin Microbiol 37(8):2543–2547PubMedGoogle Scholar
  50. Lemmer K, Donoso Mantke O, Bae HG, Groen J, Drosten C, Niedrig M (2004) External quality control assessment in PCR diagnostics of dengue virus infections. J Clin Virol 30(4):291–296PubMedCrossRefGoogle Scholar
  51. Leshem B, Sarfati G, Novoa A, Breslav I, Marks RS (2004) Photochemical attachment of biomolecules onto fibre-optics for construction of a chemiluminescent immunosensor. Luminescence 19(2):69–77PubMedCrossRefGoogle Scholar
  52. B.D. Lindenbach CMR (1996) Flaviviruses (Chapter 33). In: Knipe DM, Fields BN, Howley PM (eds) Fields virology. Lippincott-Raven, PhiladelphiaGoogle Scholar
  53. Mackay IM, Arden KE, Nitsche A (2002) Real-time PCR in virology. Nucleic Acids Res 30(6):1292–1305PubMedCrossRefGoogle Scholar
  54. Mackenzie JS, Gubler DJ, Petersen LR (2004) Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat Med 10(Suppl 12):S98–109PubMedCrossRefGoogle Scholar
  55. Marjorie Russel HBL, Clackson T (2004) Introduction to phage biology and phage display. In: Tim Clackson HBL (Ed) Phage display a practical approach, p 1–26. Oxford University Press, New YorkGoogle Scholar
  56. Marks RS, Bassis E, Bychenko A, Levine MM (1997) Chemiluminescent optical fiber immunosensor for detecting cholera antitoxin. Opt Engi 36(12):3258–3264CrossRefGoogle Scholar
  57. Mateus CFR, Huang MCY, Chang-Hasnain CJ, Foley JE, Beatty R, Li P, Cunningham BT (2004) Ultra-sensitive immunoassay using VCSEL detection system. Elect Lett 40(11)Google Scholar
  58. Megret F, Hugnot JP, Falconar A, Gentry MK, Morens DM, Murray JM, Schlesinger JJ, Wright PJ, Young P, Van Regenmortel MH et al (1992) Use of recombinant fusion proteins and monoclonal antibodies to define linear and discontinuous antigenic sites on the dengue virus envelope glycoprotein. Virology 187(2):480–491PubMedCrossRefGoogle Scholar
  59. Mukhopadhyay S, Kuhn RJ, Rossmann MG (2005) A structural perspective of the flavivirus life cycle. Nat Rev Microbiol 3(1):13–22PubMedCrossRefGoogle Scholar
  60. Murgue B, Deparis X, Chungue E, Cassar O, Roche C (1999) Dengue: an evaluation of dengue severity in French Polynesia based on an analysis of 403 laboratory-confirmed cases. Trop Med Int Health 4(11):765–773PubMedCrossRefGoogle Scholar
  61. World Health Organization (WHO) (1997) Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. Geneva, SwitzerlandGoogle Scholar
  62. World Health Organization (WHO) (2004) Dengue diagnostics. Proceedings of an international workshop, Geneva, SwitzerlandGoogle Scholar
  63. Paddle BM (1996) Biosensors for chemical and biological agents of defence interest. Biosens Bioelectron 11(11):1079–1113PubMedCrossRefGoogle Scholar
  64. Palmer CJ, King SD, Cuadrado RR, Perez E, Baum M, Ager AL (1999) Evaluation of the MRL diagnostics dengue fever virus IgM capture ELISA and the PanBio Rapid Immunochromatographic Test for diagnosis of dengue fever in Jamaica. J Clin Microbiol 37(5):1600–1601PubMedGoogle Scholar
  65. Pancharoen C, Mekmullica J, Thisyakorn U (2001) Primary dengue infection: what are the clinical distinctions from secondary infection? Southeast Asian J Trop Med Public Health 32(3):476–480PubMedGoogle Scholar
  66. Petrenko VA, Vodyanoy VJ (2003) Phage display for detection of biological threat agents. J Microbiol Methods 53(2):253–262PubMedCrossRefGoogle Scholar
  67. Petrosova TKA, Cosnier S, Trakht I, Lutwama J, Rwaguma E, Chepurnov A, Muhlberger E, Lobel L, Marks R (2007) Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa. Sensor Actuat B 122(2):578–86CrossRefGoogle Scholar
  68. Phuong CX, Nhan NT, Kneen R, Thuy PT, van Thien C, Nga NT, Thuy TT, Solomon T, Stepniewska K, Wills B (2004) Clinical diagnosis and assessment of severity of confirmed dengue infections in Vietnamese children: is the world health organization classification system helpful? Am J Trop Med Hyg 70(2):172–179PubMedGoogle Scholar
  69. Polyak B, Bassis E, Novodvorets A, Belkin S, Marks RS (2000) Optical fiber bioluminescent whole-cell microbial biosensors to genotoxicants. Water Sci Technol 42(1–2):305–311Google Scholar
  70. Polyak B, Bassis E, Novodvorets A, Belkin S, Marks RS (2001) Bioluminescent whole cell optical fiber sensor to genotoxicants: system optimization. Sensor Actuat B – Chem 74(1–3):18–26CrossRefGoogle Scholar
  71. Sabchareon A, Lang J, Chanthavanich P, Yoksan S, Forrat R, Attanath P, Sirivichayakul C, Pengsaa K, Pojjaroen-Anant C, Chambonneau L, Saluzzo JF, Bhamarapravati N (2004) Safety and immunogenicity of a three dose regimen of two tetravalent live-attenuated dengue vaccines in five- to twelve-year-old Thai children. Pediatr Infect Dis J 23(2):99–109PubMedCrossRefGoogle Scholar
  72. Salama K, Eltoukhy H, Hassibi A, El-Gamal A (2004) Modeling and simulation of luminescence detection platforms. Biosens Bioelectron 19(11):1377–1386PubMedCrossRefGoogle Scholar
  73. Sang CT, Cuzzubbo AJ, Devine PL (1998a) Evaluation of a commercial capture enzyme-linked immunosorbent assay for detection of immunoglobulin M and G antibodies produced during dengue infection. Clin Diagn Lab Immunol 5(1):7–10PubMedGoogle Scholar
  74. Sang CT, Hoon LS, Cuzzubbo A, Devine P (1998b) Clinical evaluation of a rapid immunochromatographic test for the diagnosis of dengue virus infection. Clin Diagn Lab Immunol 5(3):407–409PubMedGoogle Scholar
  75. Shu PY, Huang JH (2004) Current advances in dengue diagnosis. Clin Diagn Lab Immunol 11(4):642–50PubMedGoogle Scholar
  76. Shu PY, Chang SF, Kuo YC, Yueh YY, Chien LJ, Sue CL, Lin TH, Huang JH (2003) Development of group- and serotype-specific one-step SYBR green I-based real-time reverse transcription-PCR assay for dengue virus. J Clin Microbiol 41(6):2408–2416PubMedCrossRefGoogle Scholar
  77. Smith GP (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228(4705):1315–1317PubMedCrossRefGoogle Scholar
  78. Sobarzo A, Paweska JT, Herrmann S, Amir T, Marks RS, Lobel L (2007) Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans. J Virol Methods 146(1–2):327–334PubMedCrossRefGoogle Scholar
  79. Talarmin A, Labeau B, Lelarge J, Sarthou JL (1998) Immunoglobulin A-specific capture enzyme-linked immunosorbent assay for diagnosis of dengue fever. J Clin Microbiol 36(5):1189–1192PubMedGoogle Scholar
  80. Teles FR, Prazeres DM, Lima-Filho JL (2005) Trends in dengue diagnosis. Rev Med Virol 15(5):287–302PubMedCrossRefGoogle Scholar
  81. Thein S, Aung MM, Shwe TN, Aye M, Zaw A, Aye K, Aye KM, Aaskov J (1997) Risk factors in dengue shock syndrome. Am J Trop Med Hyg 56(5):566–572PubMedGoogle Scholar
  82. Trirawatanapong T, Chandran B, Putnak R, Padmanabhan R (1992) Mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody. Gene 116(2):139–150PubMedCrossRefGoogle Scholar
  83. Vaughn DW, Nisalak A, Kalayanarooj S, Solomon T, Dung NM, Cuzzubbo A, Devine PL (1998) Evaluation of a rapid immunochromatographic test for diagnosis of dengue virus infection. J Clin Microbiol 36(1):234–238PubMedGoogle Scholar
  84. Vaughn DW, Nisalak A, Solomon T, Kalayanarooj S, Nguyen MD, Kneen R, Cuzzubbo A, Devine PL (1999) Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections. Am J Trop Med Hyg 60(4):693–698PubMedGoogle Scholar
  85. Vaughn DW, Green S, Kalayanarooj S, Innis BL, Nimmannitya S, Suntayakorn S, Endy TP, Raengsakulrach B, Rothman AL, Ennis FA, Nisalak A (2000) Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J Infect Dis 181(1):2–9PubMedCrossRefGoogle Scholar
  86. Wu SJ, Hanson B, Paxton H, Nisalak A, Vaughn DW, Rossi C, Henchal EA, Porter KR, Watts DM, Hayes CG (1997) Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virus. Clin Diagn Lab Immunol 4(4):452–457PubMedGoogle Scholar
  87. Wu SJ, Lee EM, Putvatana R, Shurtliff RN, Porter KR, Suharyono W, Watts DM, King CC, Murphy GS, Hayes CG, Romano JW (2001a) Detection of dengue viral RNA using a nucleic acid sequence-based amplification assay. J Clin Microbiol 39(8):2794–2798PubMedCrossRefGoogle Scholar
  88. Wu HC, Huang YL, Chao TT, Jan JT, Huang JL, Chiang HY, King CC, Shaio MF (2001b) Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients. J Clin Microbiol 39(3):977–982PubMedCrossRefGoogle Scholar
  89. Wu HC, Jung MY, Chiu CY, Chao TT, Lai SC, Jan JT, Shaio MF (2003) Identification of a dengue virus type 2 (DEN-2) serotype-specific B-cell epitope and detection of DEN-2-immunized animal serum samples using an epitope-based peptide antigen. J Gen Virol 84(Pt 10):2771–2779PubMedCrossRefGoogle Scholar
  90. Wu TZ, Su CC, Chen LK, Yang HH, Tai DF, Peng KC (2005) Piezoelectric immunochip for the detection of dengue fever in viremia phase. Biosens Bioelectron 21(5):689–695PubMedCrossRefGoogle Scholar
  91. Yamada K, Takasaki T, Nawa M, Kurane I (2002) Virus isolation as one of the diagnostic methods for dengue virus infection. J Clin Virol 24(3):203–209PubMedCrossRefGoogle Scholar
  92. Zaytseva NV, Montagna RA, Baeumner AJ (2005a) Microfluidic biosensor for the serotype-specific detection of dengue virus RNA. Anal Chem 77(23):7520–7527PubMedCrossRefGoogle Scholar
  93. Zaytseva NV, Montagna RA, Baeumner AJ (2005b) Microfluidic biosensor for the serotype-specific detection of Dengue virus RNA. Anal Chem 77(23):7520–7527PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Evgeni Eltzov
    • 1
  • Danit Atias
    • 2
    • 3
  • Levi Gheber
    • 3
  • Robert S. Marks
    • 3
    • 4
    • 5
  1. 1.Unit of Environmental Engineering, Faculty of Engineering ScienceBen-Gurion University of the NegevBeer-ShevaIsrael
  2. 2.Department of Virology, Faculty of Health SciencesBen-Gurion University of the NegevBeer-ShevaIsrael
  3. 3.Department of Biotechnology EngineeringBen-Gurion University of the NegevBeer-ShevaIsrael
  4. 4.The National Institute for Biotechnology in the NegevBen-Gurion University of the NegevBeer-ShevaIsrael
  5. 5.The Ilse Katz, Center for Meso and Nanoscale Science and TechnologyBen-Gurion University of the NegevBeer-ShevaIsrael

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