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Growth Suppression of HL60 and L6 Cells by Atomic Hydrogen

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 16))

Abstract

We previously reported electrolyzed reduced water (ERW) which produced near the cathode by electrolysis has a reductive activity. We also revealed that ERW contains both molecular hydrogen and platinum nanoparticles (Pt nps) derived from platinum-coated titanium electrodes. Pt nps have catalysis activity converting molecular hydrogen to atomic hydrogen. Thus, we regard a solution containing both dissolved hydrogen and Pt nps as a model water of ERW (H2/Pt nps solution), and then we tried to examine the physiological effects of H2/Pt nps solution. To our knowledge, there is no report on physiological effects of H2/Pt nps solution. Here, we report the effects of H2/Pt nps solution on the growth of promyelocytic cell line, HL60 cells and myogenic cell lines, L6 cells. H2/Pt nps solution suppressed cell growth in the presence of both hydrogen and Pt nps in a dose dependent manner. The result of sub-G1 analysis suggests that H2/Pt nps solution induces apoptosis in both HL60 and L6 cells. The labeled Pt nps in HL60 cells were detected in liposome, suggesting Pt nps is incorporated by endocytosis. These results suggest that atomic hydrogen catalyzed by Pt nps induced significant cell growth suppression and atomic hydrogen may be one of new signal of cell function.

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References

  1. Shirahata, S., Kabayama, S., Nakano, M., Miura, T., Kusumoto, K., Gotoh, M., Hayashi, H., Otsubo, K., Morisawa, S., Katakura, Y. (1997). Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biochem. Biophys. Res. Commun. 234: 269–274.

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Authors and Affiliations

  1. Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka, 812-8581, Japan

    Kensuke Nakanishi, Takeki Hamasaki, Takuro Nakamura & Masumi Abe

  2. Graduate School of Systems Life Sciences, Kyushu University, Higashi-ku, Fukuoka, 812-8581, Japan

    Kiichiro Teruya

  3. Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Fukuoka, 812-8581, Japan

    Kiichiro Teruya

  4. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan

    Kiichiro Teruya

  5. Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka, 812-8581, Japan

    Yoshinori Katakura & Sanetaka Shirahata

  6. Graduate School of System Life Science, Kyushu University, Fukuoka, 812-0053, Japan

    Yoshinori Katakura

  7. Department of Genetic Resources Technology, Kyushu University, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  8. Nihon Trim co. LTD, Osaka, Osaka, 531-0076, Japan

    Shinkatsu Morisawa

Authors
  1. Kensuke Nakanishi
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  2. Takeki Hamasaki
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  3. Takuro Nakamura
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  4. Masumi Abe
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  5. Kiichiro Teruya
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  6. Yoshinori Katakura
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  7. Shinkatsu Morisawa
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  8. Sanetaka Shirahata
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Corresponding author

Correspondence to Sanetaka Shirahata .

Editor information

Editors and Affiliations

  1. Fac. Engineering, Dept. Chemical Engineering, Kyushu University, Motooka 744, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

  2. Fac. Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Yoshinori Katakura

  3. Fac. Engineering, Kyushu University, Fukuoka, 819-0395, Japan

    Akira Ito

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© 2010 Springer Science+Business Media B.V.

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Nakanishi, K. et al. (2010). Growth Suppression of HL60 and L6 Cells by Atomic Hydrogen . In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_53

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