Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level

Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 16)


Electrolyzed reduced water (ERW) containing hydrogen molecules and Pt nanoparticles is expected as a new antioxidant. Thiobarbituric acid reactive substances (TBARS) assay revealed that ERW had an inhibitory effect on the oxidation of low-density lipoproteins (LDL). In addition, ERW significantly suppressed LDL oxidation in the medium cultured J.774.A1 macrophage like cells. ERW inhibited Cu2+ ion-catalyzed oxidation of LDL ex vivo. ERW lowered lipid peroxide level in blood red cells and plasma triglyceride in rats fed a basal diet containing 2% cholesterol. These results suggest that ERW has anti-LDL oxidation and anti-hyperlipidemia effects.


Electrolyzed reduced water Hyperlipidemia LDL Oxidation 


  1. 1.
    Ye J., Li Y.-P., Hamsaki T., Nakamichi N., Komatsu T., Kashiwagi T., Teruya K., Nishikawa R., Kawahara T., Osada K., Toh K., Abe M., Tian H., Kabayama S., Otsubo K., Morisawa S., Katakura Y., and Shirahata S. (2008) Inhibitory effect of electrolyzed reduced water on tumor angiogenesis. Biol. Pharm. Bull. 31: 19–26.CrossRefPubMedGoogle Scholar
  2. 2.
    Ihm S.H., Yoo H.J., Park S.W., and Ihm J. (1999) Effect of aminoguanidine on lipid peroxidation in streptozotocin-induced diabetic rats. Metabolism 48: 1141–1145.Google Scholar
  3. 3.
    Yagi K. (1976) A simple fluorometric assay for lipoprotein in blood plasma. Biochem Med. 15: 212–216.CrossRefPubMedGoogle Scholar
  4. 4.
    Ohkawa H., Ohishi N., and Yagi K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95: 351–358.CrossRefPubMedGoogle Scholar
  5. 5.
    Iwai K., Nakaya N., Kawasaki Y., and Matsue H. (2002) The inhibitory effect of natto, a kind of fermented soybeans, on LDL oxidation in vitro. J. Agric. Food Chem. 50: 3592–3596.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Genetic Resources TechnologyKyushu UniversityFukuokaJapan
  2. 2.Faculty of AgricultureKyushu UniversityHigashi-kuJapan
  3. 3.Nihon Trim Co., Ltd.OsakaJapan
  4. 4.Functional Water Cell Analysis Center Co. Ltd.FukuokaJapan
  5. 5.Graduate School of Systems Life SciencesKyushu UniversityHigashi-kuJapan
  6. 6.Department of Genetic Resources Technology, Faculty of AgricultureKyushu UniversityFukuokaJapan
  7. 7.Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan
  8. 8.Graduate School of System Life ScienceKyushu UniversityFukuokaJapan
  9. 9.Department of Genetic Resources TechnologyKyushu UniversityFukuokaJapan
  10. 10.Nihon Trim co. LTDOsakaJapan

Personalised recommendations