Abstract
Short-chain fatty acids (SCFAs), acetate, propionate and butyrate, are the major anions in the lumen of the large intestine. One of the G protein coupled receptors, GPR43, was recently described as a receptor for SCFAs. To understand pharmacological role of butyrate through GPR43, we analyzed cell shape, GPR43 mRNA expression and mitogen-activated protein (MAP) kinase activation in rat hepatic stem-like (HSL) cells. RT-PCR demonstrated that GPR43 mRNA was expressed in HSL cells and the expression level was not affected by butyrate treatment. Western blot analysis revealed that ERK phosphorylation was enhanced 10 min after sodium butyrate treatment then decreased to the initial level after 24 h. In contrast, Ark phosphorylation was not detected. The results obtained were contrary to our intention, because much cell death was observed after sodium butyrate treatment.
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References
Briscoe, C.P., Tadayyon, M., Andrews, J.L., et al. (2003) The orphan G protein-coupled receptor GPR40 is activated by medium and long chain fatty acids. J. Biol. Chem. 278: 11303–11311.
Le Poul, E., Loison, C., Strnyf, S., et al. (2003) Functional characterization of human receptors fro short chain fatty acids and their role in polymorphonuclear cell activation. J. Biol. Chem. 278: 25481–25489.
Nagai, H., Terada, K., Watanabe, G., et al. (2002) Differentiation of liver epithelial (stem-like) cells into hepatocytes induced by coculture with hepatic stellate cells. Biochem Biophys. Res. Commun. 293: 1420–1425.
Nilsson, N.E., Kotarsky, K., Owman, C., et al. (2003) Identification of a free fatty acid receptor, FFA2R, expressed on leukocytes and activated by short-chain fatty acids. Biochem. Biophys. Res. Commun. 303: 1047–1052.
Riester, D., Hildmann, C., and Schwienhorst, A. (2007) Histone deacetylase inhibitors turning epigenic mechanisms of gene regulation into tools of therapeutic intervention in malignant and other diseases. Appl. Microbiol. Biotechnol. 75: 499–514.
Saheki, T., Ito, H., Sekiguchi, A., et al. (2008) Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation. Cytotechnol. 57:137–143.
Yamada, S., Terada, K., Ueno, Y., et al. (2005) Differentiation of adult hepatic stem-like cells into pancreatic endocrine cells. Cell Transplant. 14: 647–653.
Yonezawa, T., Kobayashi, Y., and Obara, Y. (2007) Short-chain fatty acids induce acute phosphorylation of the p38 mitogen-activated protein kinase/heat shock protein 27 pathway via GPR43 in the MCF-7 human breast cancer cell line. Cell Signal. 19: 185–193.
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Saheki, T. et al. (2010). Study of Butyrate Signal Transduction Pathways in Rat Hepatic Stem-Like Cells. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_40
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DOI: https://doi.org/10.1007/978-90-481-3892-0_40
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