Advertisement

Role of Ubiquitination Promoted During Restimulation in the Induction of T Cell Anergy

  • Yohei Mizuguchi
  • Ai Yamamoto
  • Makoto Hattori
  • Tadashi Yoshida
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 16)

Abstract

Recent studies have demonstrated that some E3 ubiquitin ligases were upregulated in anergic T cells, and suggested that the ubiquitination and degradation of intracellular proteins are involved in the T cell anergy. We examined in this study the kinetics of the ubiquitination in the induction of anergy. Ubiquitinated proteins in the anergic cells remarkably increased after the restimulation. Our results suggest that degradation of specific proteins would occur after the restimulation but not during the induction.

Keywords

Food Allergy Intracellular Protein Ubiquitinated Protein Immunological Tolerance Cell Anergy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Melamed, D. and Friedman, A. (1993) Direct evidence for anergy in T lymphocytes tolerized by oral administration of ovalbumin. Eur. J. Immunol. 23: 935–942.CrossRefGoogle Scholar
  2. 2.
    Heissmeyer, V., Macian, F., Im, S.H., Varma, R., Feske, S., Venuprasad, K., Gu, H., Liu, Y.C., Dustin, M.L., and Rao, A. (2004) Calcineurin imposes T cell unresponsiveness through targeted proteolysis of signaling proteins. Nat. Immunol. 5: 255–265.CrossRefGoogle Scholar
  3. 3.
    Seroogy, C.M., Soares, L., Ranheim, E.A., Su, L., Holness, C., Bloom, D., and Fathman, C.G. (2004) The gene related to anergy in lymphocytes, an E3 ubiquitin ligase, is necessary for anergy induction in CD4 T cells. J. Immunol. 173: 79–85.Google Scholar
  4. 4.
    Jeon, M.S., Atfield, A., Venuprasad, K., Krawczyk, C., Sarao, R., Elly, C., Yang, C., Arya, S., Bachmaier, K., Su, L., Bouchard, D., Jones, R., Grouski, M., Ohashi, P., Wada, T., Bloom, D., Fathman, C.G., Liu, Y.C., and Penninger, J.M. (2004) Essential Role of the E3 ubiquitin ligase Cbl-b in T cell anergy induction. Immunity 21: 167–177.CrossRefGoogle Scholar
  5. 5.
    Anandasabapathy, N., Ford, G.S., Bloom, D., Holness, C., Paragas, V., Seroogy, C., Skrenta, H., Hollenhorst, M., Fathman, C.G., and Soares, L. (2003) GRAIL: An E3 ubiquitin ligase that inhibits cytokine gene transcription is expressed in anergic CD4+ T cells. Immunity 18: 535–547.CrossRefPubMedGoogle Scholar
  6. 6.
    Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.CrossRefGoogle Scholar
  7. 7.
    Ebihara, M., Hattori, M., and Yoshida, T. (2007) Distinctly different sensitivity in the induction and reversal of anergy of Th1 and Th2 cells. Biosci. Biotechnol. Biochem. 71: 130–137.CrossRefGoogle Scholar
  8. 8.
    Andris, A., Denanglaire, S., de Mattia, F., Urbain, J., and Leo, O. (2004) Naïve T cells are resistant to anergy induction by anti-CD3 antibodies. J. Immunol. 173: 3201–3208.Google Scholar
  9. 9.
    Fang, D., Elly, C., Gao, B., Fang, N., Altman, Y., Joazeiro, C., Hunter, T., Copeland, N., Jenkins, N., and Liu, Y.C. (2002) Dysregulation of T lymphocyte function in itchy mice: A role for Itch in TH2 differentiation. Nat. Immunol. 3: 281–287.CrossRefGoogle Scholar
  10. 10.
    Yamamoto, T., Hattori, M., and Yoshida, T. (2007) Induction of T-cell activation or anergy determined by the combination of intensity and duration of T-cell receptor stimulation, and sequential induction in an individual cell. Immunology 121: 383–391.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yohei Mizuguchi
    • 1
  • Ai Yamamoto
    • 1
  • Makoto Hattori
    • 1
  • Tadashi Yoshida
    • 1
  1. 1.Department of Applied Biological ScienceTokyo University of Agriculture and TechnologyFuchuJapan

Personalised recommendations