Molecular Cloning and Characterization of a Novel Canine Sulfotransferase

  • Katsuhisa Kurogi
  • Yoichi Sakakibara
  • Shin Yasuda
  • Ming-Cheh Liu
  • Masahito Suiko
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 16)

Abstract

Sulfonation, as mediated by the cytosolic sulfotransferases (SULTs), represents an important mechanism in vivo for the detoxification/metabolism of drugs and other xenobiotics, as well as endogenous neurotransmitters and hormones. Madin-Darby canine kidney (MDCK) cells have been widely used as a model system for investigating the excretion/detoxification mechanism of kidney. In this study, a cDNA encoding a novel canine SULT was cloned from MDCK cells using reverse transcription-polymerase chain reaction (RT-PCR) technique. Nucleotide sequence revealed that the newly cloned canine SULT cDNA has an open reading frame encompassing 912 nucleotides and encoding a 303-amino acid polypeptide. Analysis of the deduced amino acid sequence showed that the novel canine SULT is highly homologous to human SULT1C4, with 82.8% amino acid identity. Recombinant canine SULT1C4, expressed and purified using the pGEX-4T-1 GST gene fusion system, exhibited strong phenol-sulfonating activities toward 1- or 2-naphthol and o-bromophenol among a variety of xenobiotic compounds tested as substrates. These data indicated that the newly identified canine SULT1C4 may function in kidney for the detoxification of xenobiotic compounds.

Keywords

MDCK Cell Amino Acid Sequence Identity Xenobiotic Compound Canine Tissue Phenol Sulfotransferases 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (B), (C) (M.S. and Y.S.), a Grant-in Aid for Encouragement of Young Scientists (Y.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Health and Sciences Research Grants (Toxicogenomics) from the Ministry of Health, Labor and Welfare of Japan (Y.S.) and start-up funds from College of Pharmacy, The University of Toledo (M.C.L.). We also thank Satoshi Miyazaki, Saki Takahashi, Dr Madhyasha Harish Kumar for great help of this study and manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Katsuhisa Kurogi
    • 1
  • Yoichi Sakakibara
    • 1
  • Shin Yasuda
    • 2
  • Ming-Cheh Liu
    • 3
  • Masahito Suiko
    • 1
  1. 1.Department of Biochemistry and Applied BiosciencesUniversity of MiyazakiMiyazakiJapan
  2. 2.Department of Bioscience, School of AgricultureTokai UniversityAsoJapan
  3. 3.Department of Pharmacology, College of PharmacyThe University of ToledoToledoUSA

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