Magnetic Cell-Patterning for Tissue Engineering

  • Hirokazu Akiyama
  • Akira Ito
  • Yoshinori Kawabe
  • Masamichi Kamihira
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 16)

Abstract

We report a magnetic cell-patterning technique to control arrangements of target cells for the fabrication of three-dimensional (3D) tissue constructs. The cells labeled with magnetite cationic liposomes (MCLs) could be attracted to the magnetic source. When the magnetic field concentrators were placed underneath the culture substrates, micro-line patterning of human umbilical vein endothelial cells (HUVECs) labeled with MCLs was formed on a monolayer of cells or a magnetic tissue-engineered cell sheet constructed by accumulating MCL-labeled cells uniformly and three-dimensionally to the culture surface using magnetic force. Based on these results, we successfully created multilayered C2C12 cell sheets, where the micro-patterned HUVECs were embedded, by alternating the processes of magnetic accumulation of C2C12 cells for cell layer formation and magnetic patterning of HUVECs onto the cell layers. These results suggested that this cell-patterning technique could be a useful tool for tissue engineering.

Keywords

Steel Plate Human Umbilical Vein Endothelial Cell C2C12 Cell Cell Sheet Cell Patterning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Brock, A., Chang, E., Ho, C.C., LeDuc, P., Jiang, X., Whitesides, G.M., and Ingber, D.E. (2003) Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir 19: 1611–1617.CrossRefPubMedGoogle Scholar
  2. 2.
    Hui, E.E. and Bhatia, S.N. (2007) Micromechanical control of cell-cell interactions. Proc. Natl. Acad. Sci. U S A. 104: 5722–5726.CrossRefPubMedGoogle Scholar
  3. 3.
    Ito, A., Akiyama, H., Kawabe, Y., and Kamihira, M. (2007) Magnetic force-based cell patterning using Arg-Gly-Asp (RGD) peptide-conjugated magnetite cationic liposomes. J. Biosci. Bioeng. 104: 288–293.CrossRefPubMedGoogle Scholar
  4. 4.
    Ito, A., Hayashida, M., Honda, H., Hata, K., Kagami, H., Ueda, M., and Kobayashi, T. (2004) Construction and harvest of multilayered keratinocyte sheets using magnetite nanoparticles and magnetic force. Tissue Eng. 10: 873–880.CrossRefPubMedGoogle Scholar
  5. 5.
    Ino, K., Ito, A., and Honda, H. (2007) Cell patterning using magnetite nanoparticles and magnetic force. Biotechnol. Bioeng. 97: 1309–1317.CrossRefPubMedGoogle Scholar
  6. 6.
    Jiang, X., Xu, Q., Dertinger, S.K., Stroock, A.D., Fu, T.M., and Whitesides, G. M. (2005) A general method for patterning gradients of biomolecules on surfaces using microfluidic networks. Anal. Chem. 15: 2338–2347.CrossRefGoogle Scholar
  7. 7.
    Khademhosseini, A., Langer, R., Borenstein, J., and Vacanti, J.P. (2006) Microscale technologies for tissue engineering and biology. Proc. Natl. Acad. Sci. USA. 103: 2480–2487.CrossRefPubMedGoogle Scholar
  8. 8.
    Kobayashi, A., Miyake, H., Hattori, H., Kuwana, R., Hiruma, Y., Nakahama, K., Ichinose, S., Ota, M., Nakamura, M., Takeda, S., and Morita, I. (2007) In vitro formation of capillary networks using optical lithographic techniques. Biochem. Biophys. Res. Commun., 358: 692–697.CrossRefPubMedGoogle Scholar
  9. 9.
    Liu Tsang, V., Chen, A.A., Cho, L.M., Jadin, K.D., Sah, R.L., DeLong, S., West, J.L., and Bhatia, S.N. (2007) Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels. FASEB J. 21: 790–801.CrossRefPubMedGoogle Scholar
  10. 10.
    Nelson, C.M., Jean, R.P., Tan, J.L., Liu, W.F., Sniadecki, N.J., Spector, A.A., and Chen, C.S. (2005) Emergent patterns of growth controlled by multicellular form and mechanics. Proc. Natl. Acad. Sci. U S A. 16: 11571–11572.Google Scholar
  11. 11.
    Shinkai, M., Yanase, M., Honda, H., Wakabayashi, T., Yoshida, J., and Kobayashi, T. (1996) Intracellular hyperthermia for cancer using magnetite cationic liposomes: in vitro study Jpn. J. Cancer Res. 87: 1179–1183.Google Scholar
  12. 12.
    Tsuda, Y., Shimizu, T., Yamato, M., Kikuchi, A., Sasagawa, T., Sekiya, S., Kobayashi, J., Chen, G., and Okano, T. (2007) Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Biomaterials 28: 4939–4946.CrossRefPubMedGoogle Scholar
  13. 13.
    Wilson, W.,C., Jr., and Boland, T. (2003) Cell and organ printing 1: protein and cell printers. Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 272: 491–496.CrossRefPubMedGoogle Scholar
  14. 14.
    Xu, T., Gregory, C. A., Molnar, P., Cui, X., Jalota, S., Bhaduri, S.B., and Boland, T. (2006) Viability and electrophysiology of neural cell structures generated by the inkjet printing method. Biomaterials 27: 3580–3588.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hirokazu Akiyama
    • 1
  • Akira Ito
    • 1
  • Yoshinori Kawabe
    • 1
  • Masamichi Kamihira
    • 2
  1. 1.Department of Chemical Engineering, Faculty of EngineeringKyushu UniversityFukuokaJapan
  2. 2.Graduate School of Systems Life ScienceKyushu UniversityFukuokaJapan

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