Magnetic Cell-Patterning for Tissue Engineering
We report a magnetic cell-patterning technique to control arrangements of target cells for the fabrication of three-dimensional (3D) tissue constructs. The cells labeled with magnetite cationic liposomes (MCLs) could be attracted to the magnetic source. When the magnetic field concentrators were placed underneath the culture substrates, micro-line patterning of human umbilical vein endothelial cells (HUVECs) labeled with MCLs was formed on a monolayer of cells or a magnetic tissue-engineered cell sheet constructed by accumulating MCL-labeled cells uniformly and three-dimensionally to the culture surface using magnetic force. Based on these results, we successfully created multilayered C2C12 cell sheets, where the micro-patterned HUVECs were embedded, by alternating the processes of magnetic accumulation of C2C12 cells for cell layer formation and magnetic patterning of HUVECs onto the cell layers. These results suggested that this cell-patterning technique could be a useful tool for tissue engineering.
KeywordsSteel Plate Human Umbilical Vein Endothelial Cell C2C12 Cell Cell Sheet Cell Patterning
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