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Bacterial Chromatin pp 175–201Cite as

Dps and Bacterial Chromatin

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Abstract

When Dps was first discovered in Escherichia coli it was soon realized that it is an unusual protein. The purified Dps bound efficiently, but non-specifically, to DNA forming highly ordered complexes and mutants lacking Dps were sensitive to oxidative stress. Since then Dps proteins have been found in many bacteria. Structural studies revealed that the Dps monomer forms a bundle structure resembling the iron binding ferritins and bacterioferritins and upon oligomerization it assembles into a dodecamer with a hollow core. Like ferritins Dps proteins are also able to sequester and detoxify iron through a ferroxidase center that uniquely to the Dps family is shared between two monomers. Thus, the protective capabilities of Dps rely both on its ability to bind and physically protect DNA and its ability to detoxify iron that otherwise may catalyze the production of toxic free radicals. On the other hand Dps can also be used for iron storage under iron limiting conditions. Iron restriction and oxidative stress characterizes the environment that bacterial pathogens encounter in the human host and Dps proteins are required for full virulence of several pathogens. Thus, Dps is a versatile protein that at multiple levels protects bacterial cells against stress.

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Acknowledgements

I am deeply grateful to Prof. Abraham Minsky for providing the micrographs displayed in Fig. 9.2 and for the helpful comments on the manuscript.

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Authors and Affiliations

  1. Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 4, DK-1870, Frederiksberg C, Denmark

    Hanne Ingmer

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  1. Hanne Ingmer
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Correspondence to Hanne Ingmer .

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  1. Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC, Leiden, Netherlands

    Remus T. Dame (Faculty of Mathematics and Natural Sciences) (Faculty of Mathematics and Natural Sciences)

  2. Division of Physics and Astronomy Section Physics of Complex Systems, VU University Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands

    Remus T. Dame (Faculty of Mathematics and Natural Sciences) (Faculty of Mathematics and Natural Sciences)

  3. Department of Microbiology School of Genetics and Microbiology, University of Dublin Trinity College, Dublin 2, Ireland

    Charles J. Dorman

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Ingmer, H. (2010). Dps and Bacterial Chromatin. In: Dame, R.T., Dorman, C.J. (eds) Bacterial Chromatin. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3473-1_9

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