Abstract
We have evaluated various DNA methyl transferase inhibitors (iDNMTs) and histone deacetylase inhibitors (iHDACs) for their effect on transient gene expression in mammalian cells. The inhibitors were selected on the basis of differences in their reported mechanism of action or cellular activities. The optimal concentrations of the inhibitors were determined and their activities were compared in HEK293E and CHO-DG44 cells transiently expressing a recombinant antibody. Their effects on transient gene expression from different vectors were also evaluated.
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Backliwal, G., Hildinger, M., Küttel, I., Hacker, D.L., Wurm, F.M. (2010). Optimization and Comparison of Different DNA Methyl Transferase and Histone Deacetylase Inhibitors for Enhancing Transient Protein Expression. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_45
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DOI: https://doi.org/10.1007/978-90-481-3419-9_45
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