Transcriptomic Analysis of Antibody Producing NS0 Cell Line Under Hypothermic and Hypoxic Conditions

Abstract

Hypothermia and hypoxia were shown to reduce proliferation rate and arrest cells in the G2 phase of the cell cycle. However, in contrast to hypoxia a slight increase in total antibody production was observed at suboptimal temperature. Both hypothermia and hypoxia led to specific changes in gene expression. Hypothermia induced unique changes within a wide range of genes involved in metabolic and signaling pathways. Most deregulated genes involved in essential metabolic pathways (i.e. glycolysis/gluconeogenesis, pentose phosphate pathway and inositol metabolism) were repressed in cells cultured at 22°C. Under hypoxia the changes included the up-regulation of pathways such as glycolysis that ultimately led to alternative routes of ATP generation and increased oxygen availability, and down-regulation of genes involved in purine/pyrimidine metabolism as well as one carbon pool metabolism required for RNA and DNA synthesis.