New Disposable Fixed-Bed Bioreactor for Cell Culture and Virus Production Based on a Proprietary Agitation and Aeration System
Nowadays, there are very few efficient disposable solutions for viral vectors and vaccines manufacturing. To fill this unmet need, we developed a novel scalable fixed-bed disposable bioreactor efficient for high yield virus production. Here we present the concept validation with an animal vaccine system (MDBK cells with BHV virus). In such fixed-bed reactor of 500 ml of packed-bed (identical footprint to a 2 L bench-scale reactor), the production is comparable to what can be obtained in 32 cell-factories of 6,320 cm2 (CF-10) or in a 10 L bioreactor microcarrier cultivation on Cytodex-1 at 6 g/L.
KeywordsBioreactor Disposable Fixed-bed Packed-bed Vaccine
Cells and virus were kindly provided by Prof. E. Thiry (Lab. of Veterinary Virology, ULg, Liège, Belgium)
This work was supported by a grant of the Brussels-Capitale Region (Belgium).