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New Disposable Fixed-Bed Bioreactor for Cell Culture and Virus Production Based on a Proprietary Agitation and Aeration System

  • J.-C. DrugmandEmail author
  • N. Havelange
  • S. Osumba
  • F. Bosco
  • F. Debras
  • F. Collignon
  • E. Mathieu
  • J. Castillo
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 4)

Abstract

Nowadays, there are very few efficient disposable solutions for viral vectors and vaccines manufacturing. To fill this unmet need, we developed a novel scalable fixed-bed disposable bioreactor efficient for high yield virus production. Here we present the concept validation with an animal vaccine system (MDBK cells with BHV virus). In such fixed-bed reactor of 500 ml of packed-bed (identical footprint to a 2 L bench-scale reactor), the production is comparable to what can be obtained in 32 cell-factories of 6,320 cm2 (CF-10) or in a 10 L bioreactor microcarrier cultivation on Cytodex-1 at 6 g/L.

Keywords

Bioreactor Disposable Fixed-bed Packed-bed Vaccine 

Notes

Acknowledgments

Cells and virus were kindly provided by Prof. E. Thiry (Lab. of Veterinary Virology, ULg, Liège, Belgium)

This work was supported by a grant of the Brussels-Capitale Region (Belgium).

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • J.-C. Drugmand
    • 1
    Email author
  • N. Havelange
    • 1
  • S. Osumba
    • 1
  • F. Bosco
    • 1
  • F. Debras
    • 1
  • F. Collignon
    • 1
  • E. Mathieu
    • 1
  • J. Castillo
    • 1
  1. 1.Artelis SABrusselsBelgium

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